Heteronuclear dipole-to-dipole cross-relaxation has been applied to exploring intermolecular interactions and intramolecular spatial proximities in a large supramolecular structure comprised of a beta-barrel membrane protein, OmpX, in complex with a polymeric surfactant, amphipol A8-35. The experiments, performed in either the laboratory or the rotating frame, reveal the existence of intermolec...

doi:10.1002/2211-5463.12410 Mitochondrial uncoupling protein-2 (UCP2) mediates free fatty acid (FA)dependent H translocation across the inner mitochondrial membrane (IMM), which leads to acceleration of respiration and suppression of mitochondrial superoxide formation. Redox-activated mitochondrial phospholipase A2 (mt-iPLA2c) cleaves FAs from the IMM and has been shown to acts in synergy with ...

The intensity of free-radical processes in the skeletal muscles rats at different dietary supply with protein and sucrose was studied. It has been established that most pronounced intensification free radical mitochondria is found animals kept on a low-protein/ high-sucrose diet. In particular, generation superoxide anion-radical increases more than 5,3-fold hydroxyl 3,2-fold compared control, ...

Background: Spinal cord injury (SCI) is a traumatic or nontraumatic injury that can leads to loss of functions such as feeling or mobility along with permanent and debilitating influences on the patient’s quality of life. Patients with SCI have weakened sexual function and a distinctive sperm profile described by normal to high sperm concentrations and unusually low sperm mobility. The reason o...

Protein structures are stabilized by a variety of noncovalent interactions (NCIs), including the hydrophobic effect, hydrogen bonds, electrostatic forces and van der Waals' interactions. Our knowledge of the contributions of NCIs, and the interplay between them remains incomplete. This has implications for computational modeling of NCIs, and our ability to understand and predict protein structu...

BACKGROUND AND AIM Spontaneous bacterial peritonitis (SBP) is a major complication of liver cirrhosis and accounts for significant mortality. Although oxygen free radicals and nitric oxide been implicated in the pathophysiology of liver cirrhosis, information on their role during the development of SBP is scarce. This study examined these active species in ascitic fluid from patients with SBP, ...

Protein carbonyl (PC) and malonaldehyde (MA) contents and protein extractability (PE) in yellowtail meat containing NaCl stored at -20 degrees C for 20 weeks were analyzed. Although the PC and MA contents increased in all samples, the PE value decreased in the samples containing NaCl. After 20 weeks of storage, the protein (approximately 97.4 kDa) disappeared in the samples containing NaCl.

AIMS Chronic alcohol exposure leads to a deficiency of group B vitamins and increased risk of alcoholic cardiomyopathy characterized by impaired ventricular contractility. This study was designed to examine the effect of group B vitamin supplementation on short-term exposure of the main alcohol metabolite acetaldehyde (ACA)-induced cardiac contractile dysfunction in rat ventricular myocytes. ...