The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied to genome-wide screens. Here, we compare the efficacy of genome-wide CRISPR/Cas9-mediated forward genetic screens versus gene-trap...

The zebrafish is proving to be highly amenable to in vivo small molecule screening. With a growing number of screens successfully completed, a rich interface is being created between disciplines that have historically used zebrafish (e.g., embryology and genetics) and disciplines focused on small molecules (e.g., chemistry and pharmacology). Navigating this interface requires consideration of t...

BACKGROUND Emergency Department (ED) care has been reported to be prone to patient safety incidents (PSIs). Improving our understanding of PSIs is essential to prevent them. A standardized, peer review process was implemented to identify and analyze ED PSIs. The primary objective of this investigation was to characterize ED PSIs identified by the peer review process. A secondary objective was t...

The increasing array of strategies and models for improving clinical practice and patient outcomes can be confusing for clinicians. The Clinical Support Systems (CSS) model has proved to be effective in local environments because it demystifies the design and implementation of evidence-based practice improvement projects. The CSS model is simple and has a wide scope. It provides a broad framewo...

Over the years, the film industry has seen cyclical changes in the range of screen sizes, some dictated by film format, and some by exhibition economics. The small screens of the 1930s were eclipsed by the large-format screens of the 1950s; today, the “pillbox” screens of the 1970s are being supplanted by relatively huge screens for 35 mm film projection. The impact of large screens for audienc...

A high-gain screen can be made as a sandwich of a hologram and a retroreflective screen material. When a hologram is used in front of the screen instead of the screen material alone, the position of high-brightness viewing can be moved to any desired angle rather than being directed back along the projection beam.