introduction: proteolytic enzymes, specially collagenase, are used to digest extracellular matrix, cells isolation and primary culture. it is important to find new sources of plant or animal protease instead of bacterial or tissue collagenase. in the present research, actinidin, a plentiful protease in kiwifriut, was used to isolate human umbilical vein endothelial cells. materials and methods:...

Spent mushroom substrate (SMS) from Pleurotus ostreatus is residue of cultivation that known to have lignocellulolytic enzyme activity in it. Enzyme activities provides information on evidence fungi converting complex substrates simple ones as nutrients for them. In this study, the was found cellulase, lignin peroxidase, and protease. The samples used consisted three SMS were collected Padang P...

Three proteolytic enzymes have been isolated from sporulating cultures of Bacillus subtilis. These activities were, respectively, a protease inhibited by ethylenediaminetetraacetic acid (EDTA) but not phenylmethylsulfonyl fluoride (PMSF), a protease active on both protein and ester substrates, and an ester-active enzyme with low activity on proteins. The latter two enzymes were inhibited by PMS...

Three hundred one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets containing non-starch polysaccharide (NSP) enzyme and different levels of acid protease from 1 to 42 days of age to investigate the effects of exogenous enzymes on growth performance, digestive function, activity of endogenous digestive enzymes in the pancreas and mRNA expression of pancreatic digestive...

Three experiments were carried out to evaluate the effect of dietary combination different enzymes (phytase + protease) on performance, metabolizable energy, and amino acid digestibility broiler chickens fed diets with nutritional reduction. A total 1,400, 336, 384 male distributed in a completely randomized design, metabolism, digestibility, respectively. Treatments divided as follows: positiv...

Endophytic microbia are known as natural sources for producing valuable enzymes. In this study, four endophytic fungi were isolated from roots of local Catharanthus roseus (L.) G. Don var. (purple flower) and C. ocellatus (red stamens white widely grown in Nha Trang. They identified Fusarium solani RN1, Chaetomium funicola RN3, Penicillium rugulosum RN4 homopilatum WN1 based on morphologies col...

Proteases are the most important group of the enzymes produced commercially and industrial purpose[1,2]. They have extensive applications in a range of industrial products and processes including detergents, food, pharmaceuticals and leather[3,4]. They are classified into various groups such as alkaline protease, serine protease, cystein protease, aspartic protease and metallo protease[5]. Prot...

Extracellular protease production by Clostridium bifermentans NCTC 2914 occurred throughout the growth phase in batch culture. In both glucose-excess and -limited chemostats, protease formation was inversely related to the dilution rate, over the range D = 0.03 to 0.70 h-1. At high dilution rates (D greater than 0.25 h-1), protease activities were greatest under excess glucose conditions. Sodiu...

Guava (Psidium guajava L.), is adapted to an extensive range of soils, however, its development is strongly affected by salinity. Environmental stresses are of the major and influential factors in the amount of secondary metabolites, especially phenolic compounds present in plants. The purpose of this investigation was to study the leaf antioxidant and flavonoid contents (catechin, quercetin an...

The intracellular protease extracted from the freeze-dried mycelia obtained after the growth of Mucor pusillus at 30 degrees C in corn steep liquor medium was chromatographed on DEAE-A50. Some characteristics of the protease fractions obtained after ion-exchange chromatography were determined and compared with those of the extracellular proteases reported previously. The mycelia were found to c...