comparison with the negative control was scored as positive. Furthermore, in vitro allergy testing using the Phadia CAP system for specific IgE against shrimp, fish-mix, rainbow trout, rCyp p 1 and protamine sulfate was performed. Results: Skin prick tests were positive to shrimp, fish-mix, mackerel, salmon and protamine sulfate. Intradermal testing with protamine showed a wheal diameter of 7 m...

OBJECTIVES The purpose of this study was to determine risk factors for adverse events following protamine administration after cardiopulmonary bypass. BACKGROUND Intravenous protamine administration is associated with a risk of severe systemic reactions. However, risk factors for these events have not been well delineated, thus hampering development of preventive strategies. METHODS A case-...

An assay has been developed for specific measurement of two different lipase activities in postheparin plasma. Lipoprotein lipase, derived from extrahepatic sources, is measured as protamine-inactivated lipase activity; hepatic lipase activity is protamine-resistant under the conditions of this assay. In 100 normal subjects, both enzyme activities were noted to be related to age and sex. Protam...

We used competitive thromboxane A2-prostaglandin endoperoxide receptor blockade (SQ 30,741) as a probe to evaluate the role of thromboxane in ovine pulmonary vasoconstriction associated with protamine reversal of heparin anticoagulation. Control heparin-protamine reactions induced rapid release of thromboxane into arterial plasma (more than 1 ng/ml plasma), a 2.5-fold increase of pulmonary arte...

introduction: the aim of this study was to evaluate the effect of sperm protamine deficiency on fertilization and embryo development post icsi. material and methods: semen samples from 27 patients candidate for icsi were assessed for semen parameters. sperm processing was carried out using pure sperm and percentage of protamine deficient sperm was assessed by chromomycin a3 post processing. cor...

We report here on improved solid-contact electrochemical polyion sensors for the detection of polyion protamine. The polymeric membrane sensors were fabricated with a conducting polymer as an ion-electron transduction layer. We observed that decreasing the magnitude of the applied current pulse caused a significant improvement of the sensor sensitivity to low protamine levels. The protamine sen...

comparison with the negative control was scored as positive. Furthermore, in vitro allergy testing using the Phadia CAP system for specific IgE against shrimp, fish-mix, rainbow trout, rCyp p 1 and protamine sulfate was performed. Results: Skin prick tests were positive to shrimp, fish-mix, mackerel, salmon and protamine sulfate. Intradermal testing with protamine showed a wheal diameter of 7 m...

INTRODUCTION Currently, a dose of protamine equal to 1 mg for each 100 units of heparin given is used to reverse the residual heparin activity following off-pump coronary artery bypass. We hypothesized that a 1:1 ratio (ratio of protamine to heparin) could be higher than necessary inducing post-operative disturbance of hemostasis. METHODS Between January and March 2014 in 9 patients undergoin...

OBJECTIVE To investigate the association between smoking, semen quality, and the histone-to-protamine transition ratio in mature sperm. DESIGN Biochemical and molecular analysis in human samples and a cell line. SETTING Andrology laboratory in a university-affiliated hospital. PATIENT(S) Semen samples from 147 heavy smokers and 175 nonsmokers receiving infertility treatment. INTERVENTIO...