We prepared monoclonal antibodies against penicillin-binding protein 1B (PBP 1B) of Escherichia coli to study the membrane topology, spatial organization, and enzyme activities of this protein. The majority of the antibodies derived with PBP 1B as the immunogen reacted against the carboxy terminus. To obtain monoclonal antibodies recognizing other epitopes, we used PBP 1B lacking the immunodomi...

In this normative paper, we derive payback period (PBP) and internal rate of return (IRR) in the presence of real options. In a Kulatilaka Trigeorgis General Real Option Pricing Model, we derive the expected value of these two decision rules that corresponds to the expected NPV Bellman dynamic programming maximizing strategy in the presence of the options to wait, to mothball and to abandon. A ...

Carbon foams were synthesized from blends of coal and several C-containing additives different nature, with the aim to study their influence on foaming step, hence in final properties obtained foams. The included biomass agricultural products, thermoplastic thermosetting polymers, at concentrations, ranging 5 20 wt%. effects pore structure, carbon matrix thermal resulting investigated. presence...

Now that multi-tiered plant factories with artificial lighting (PFALs) have demonstrated sufficient proof of concept for leafy green and microgreen production; the next challenge is to determine optimal environment conditions horticultural management practices required produce nutrient-dense plant-based protein (PBP) crops within these advanced controlled systems (CES). Sole-source PFALs energe...

High-level resistance to beta-lactam antibiotics in Streptococcus pneumoniae is mediated by successive alterations in essential penicillin-binding proteins (PBPs). In the present work, single amino acid changes in S. pneumoniae PBP 2x and PBP 2b that result in reduced affinity for the antibiotic and that confer first-level beta-lactam resistance are defined. Point mutations in the PBP genes wer...

The recent emergence of group B streptococcal isolates exhibiting increased penicillin MICs at the Funabashi Municipal Medical Center and other hospitals in Japan prompted a comparative analysis of the penicillin-binding proteins (PBPs) from those strains with the PBPs from penicillin-susceptible strains comprising four neonatal invasive strains isolated from 1976 to 1988 and two recent isolate...

General odorant binding proteins (GOBPs) and pheromone binding proteins (PBPs) form a monophyletic subfamily of insect odorant binding proteins (OBPs) specific for Lepidoptera, butterflies and moths. The GOBP/PBP genes include six subgroups (GOBP1-2, PBP-A-D) previously reported to form a complex arrayed in a conserved order in representative moths (superfamily Bombycoidea) and butterflies (Nym...

Penicillin-binding protein 2 (PBP 2) has long been known to be essential for rod-shaped morphology in gram-negative bacteria, including Escherichia coli and Pseudomonas aeruginosa. In the course of earlier studies with P. aeruginosa PBP 2, we observed that E. coli was sensitive to the overexpression of its gene, pbpA. In this study, we examined E. coli overproducing both P. aeruginosa and E. co...

Extensions of the Johansson minimal logic J are investigated. It is proved that the weak interpolation property WIP is decidable over J. Well-composed logics with the Graig interpolation property CIP, restricted interpolation property IPR and projective Beth property PBP are fully described. It is proved that there are only finitely many well-composed logics with CIP, IPR or PBP; for any well-c...

Three-dimensional (3D) printing of hydrogels to form complex structures has been widely used in tissue engineering. Projection-based 3D (PBP) faster printing, lower cell damage, and higher resolution compared with other methods, providing a potential strategy for elaborate hydrogel structures. In this review, we introduced the composition process PBP. We then discuss study PBP, including chemic...