Haematococcus pluvialis (Flotow) is a unicellular green alga, which is considered to be the best astaxanthin-producing organism. Molecular markers are suitable tools for the purpose of finding out genetic variations in organisms; however there have been no studies conducted on ISSR or RAPD molecular markers for this organism. The DNA of 10 different strains of H. pluvialis (four strains from Ir...

In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...

Chicken meat is a valuable source of protein and consumption of it continues to rise day to day. The aim of the present study was to evaluate the genetic homogeneity of Listeria monocytogenes (L. monocytogenes) isolates obtained from chicken carcasses and human. Random Amplification of Polymorphic DNA (RAPD) PCR with three different primers were used to analyze the 31 L. monocytogenes isolates ...

In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed usin...

mahaleb (prunus mahalab l.) is used as a rootstock for cherries in iran and many other countries. in this research, rapd markers were used to determine the genetic diversity among eight genotypes of mahaleb evaluated in two separate research grardens in karaj, iran. these genotypes are used as mother plants for seed production. totally 40 random primers were examined for pcr reactions on the te...

DNA size markers (ladder) are essential tools in molecular biology, genetics and biotechnology. In this study, a simple and cost-effective method for laboratory production of DNA ladders is introduced. For this purpose, different sizes of 100 to 2000 bp DNA segments were designed using PCR technique. For producing 14 different gene fragments as DNA molecular weight markers, recombinant plasmid ...

The shape of the chromosomal DNA of the sulfur-dependent archaebacterium Sulfolobus acidocaldarius was analyzed by the pulsed-field gel electrophoresis(PFGE). S.acidocaldarius DNA digested with Notl showed two DNA bands at around 1.0 Mbp and 2.1 Mbp. Notl-linking clones were isolated from the library of S.acidocaldarius chromosomal DNA. It contained two Notl sites. Both 1.0 and 2.1 Mbp DNA band...

We report the detection and molecular characterization of extended spectrum β-lactamases in a series of 112 clinical isolates of Enterobacteriaceae from the Hôpital Principal de Dakar, Senegal, including five CTX-M-15-producing Morganella morganii isolates, which are reported for the first time in this country.

DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...

Two Vietnamese rice mutants (M2-2 and M2-4 second generation line 2 and line 4 respectively), induced by gamma irradiation were examined and compared to their parent (TT), and the Australian cultivar Amaroo (Am) for salt tolerance and genomic differences. Seedlings in tissue culture provided high yields and good quality DNA, especially with young shoots. Changes in DNA of the mutants in compari...