ABSTRACT Infectious RNA of wheat streak mosaic virus (WSMV) has been produced using a full-length cDNA clone as a template for in vitro transcription with SP6 RNA polymerase. Infectivity was dependent on the use of template plasmid DNA that had not undergone spontaneous rearrangement during amplification in Escherichia coli. The presence of WSMV in systemically infected wheat plants inoculated ...

The viral genome-linked protein (VPg) of Potyviruses is covalently attached to the 5' end of the genomic RNA. Towards biophysical characterization, the VPg coding region of Cardamom mosaic virus (CdMV) was amplified from the cDNA and expressed in E. coli. Most of the expressed VPg aggregated as inclusion bodies that were solubilized with urea and refolded with L-arginine hydrochloride. The vari...

Artificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically downregulate RNAs that contain complementary sequences. Transgenic plants expressing high levels of one or more amiRNAs targeting particular sequences in the genomes of some RNA viruses have shown specific resistance to the corresponding virus. This is the case of th...

Triticum mosaic virus (TriMV), an economically important virus infecting wheat in the Great Plains region of the USA, is the type species of the Poacevirus genus in the family Potyviridae. Sensitive and high-throughput serology-based detection methods are crucial for the management of TriMV and germplasm screening in wheat breeding programs. In this study, TriMV coat protein (CP) was expressed ...

The RNA silencing pathway mediated by small interfering RNAs (siRNAs) plays an important antiviral role in eukaryotes. To counteract this defense barrier, a large number of plant viruses express proteins with RNA silencing suppression activity. Recently, it was reported that the ipomovirus Cucumber vein yellowing virus (CVYV), which lacks the typical silencing suppressor of members of the famil...

Soybean mosaic virus (SMV; Genus Potyvirus; Family Potyviridae), one of the most widespread viruses in soybean (Glycine max [L.] Merr), can be effectively managed by deployment of resistance. Hutcheson, one SMV-resistant cultivar, is widely used in the MidSouth region and carries an Rsv1 allele for resistance to SMV pathotypes G1-G3, the common strains in US soybean fields. Recently, resistance...

Resistance to Soybean mosaic virus (SMV; Genus Potyvirus; Family Potyviridae) in soybean (Glycine max [L.] Merr.) is controlled by single dominant genes at three distinct loci, Rsv1, Rsv3 and Rsv4. The mechanisms of resistance at the Rsv3 locus were investigated in Harosoy and the Williams isoline L29 by tracking virus replication and movement. Two other cultivars with two genes for resistance ...

Viral determinants involved in systemic infection of hosts by monocot-infecting plant viruses are poorly understood. Wheat streak mosaic virus (WSMV, genus Tritimovirus, family Potyviridae) exclusively infects monocotyledonous crops such as wheat, oat, barley, maize, triticale, and rye. Previously, we reported that WSMV CP amino acids 36-84 are expendable for systemic infection of wheat, maize,...

In 1995, Cowpea aphid-borne mosaic virus (CABMV) of the family Potyviridae was reported to infect peanut (Arachis hypogaea L.) in Brazil (6). Leaf symptoms consisted of ring spots and blotches, and virus incidence was high when peanut was intercropped with cowpea (Vigna unguiculata (L.) Walp. subsp. unguiculata). CABMV was seed transmitted at approximately 0.15% in peanut and 6% in cowpea (6) a...

In spite of their widespread occurrence, only few host jumps by plant viruses have been evidenced and the molecular bases of even fewer have been determined. A combination of three independent approaches, 1) experimental evolution followed by reverse genetics analysis, 2) positive selection analysis, and 3) locus-by-locus analysis of molecular variance (AMOVA) allowed reconstructing the Potato ...