A flexible analytical system which allows for the continuous potentiometric monitoring of the disappearance of an electrochemical species, ferrocyanide, by the peroxidase enzyme is described. The ability of peroxidase to mediate the oxidation of indole-3-acetic acid is followed by observing the competition of indole-3-acetic acid with ferrocyanide for the peroxidase enzyme. This is accomplished...

Proton–ligand association constants of imidazole-4-acetic acid (IMA) were determined potentiometrically in aqueous solution at different temperatures in the range 15–35 C. The stepwise stability constants of IMA with some selected bivalent transition metal ions were also determined in 0.1 mol dm NaNO3. The stability of the complexes follows the trend Cu [ Ni [ Co [ Mn, which is in agreement wit...

A simple and rapid indirect potentiometric titration of sulphamethoxazole in the presence of trimethoprim contained in co-trimazole tablets is described. The method is based on the formation of a complex of sulphamethoxazole with a known excess of silver ions and the titration of unreacted silver ion potentiometrically using an inexpensive lab-made copper based mercury film electrode (CBMFE). T...

Briggs-Rauscher (BR) reaction is one of the most commonly studied oscillation reactions which has been applied for measurement of antioxidant activity of water-soluble substances. By addition of free radicals (from fruits or vegetables), there is an immediate quenching of oscillations. The reaction is followed potentiometrically and the inhibition time (IT), or time of no oscillations, is propo...

C OLORIMETRIC ANALYSIS has been employed extensively in determination of copper and zinc in human serum and other biologic materials. Greenleaf (1) and Callan and Henderson (2) determined copper colorimetrically using dithizone and diethyldithiocarbamate, respectivelv. Zinc was determined colorinietricallv by Fischer and Leopoldi (4) and by Wolf (5), using dithizone. These procedures were evalu...

The dicyclohexylcarbodiimide (DCCD)-binding proteolipid of the chloroplast ATPase complex was solubilized in 1-butanol, isolated by ion exchange chromatography, and reconstituted in a liposomal system. Proton-conducting activity was monitored by fluorometry with 9-aminoacridine as an indicator of delta pH in K+-loaded liposomes suspended in a K+-free medium. Addition of valinomycin served to cr...

One of the problems occurring after joint replacement is connected with post-surgery infections which are caused by bacteria that form biofilms on surfaces and referred to as biofilm-related (BRI). It also worth noting due bioresponse, strong pathophysiological changes in microclimate an affected surface occur (decrease pH, formation various reactive oxygen species (ROS), depletion Fe ions, inc...

In organic potato cultivation technologies, various biopreparations are used, which have a considerable impact on both the growth and development of crops, soil fertility indicators, is especially important for sod-podzolic soils characterised by low potential fertility. The purpose study to establish changes in humus content mineral nitrogen sod-mediumpodzolic loam under influence decompositio...

Purpose of the study: to reveal changes in some humus characteristics chernozems under influence two factors, specifically, humidification and flow endogenous hydrogen. Place time study. Soil sampling hydromenty were carried out on territory Gryazinsky district Lipetsk region (Krivoborgsko-Saltykovsky trough, with leached chernozems) Oktyabrsky Voronezh (Khopersky deep fault, ordinary June 2017...

The values of K~ for sulfoacetic and 3-sulfop:i:'oBionic acids were determined potentiometrically. The values are (8.6 t 0.3) x 10-~ and (3.0 t O.l) x lo-5, respectively. These values or Ka2 were much lower. than those predicted by the Branch and Calvin equation for the calculation of values or pKa of derivf.tives of acetic acid. The presence of intramolecular hydrogen bonds in sulfoacetate and...