Potato y disease caused by potato virus y (PVY) is widespread wherever potato is grown. PVY affects plants and yields. The effectiveness of extracts from garlic cloves (GE) and onion stems (OE) against potato virus y (PVY) in vitro and in vivo has been evaluated. GE and OE reduced the in vitro and in vivo infectivity of PVY to a certain extent, expressed as the number of local lesions induced b...

Potato Virus Y (PVY) is a member of the Potyviridae family – the largest plant virus family. PVY infects Solanaceae – agriculturally important species such as tobacco, potato, pepper, tomato This pathogen causes decrease in yield and quality. PVY isolates have been divided into three main strains: PVYN, PVYO and PVYC, according to the symptoms induced on tobacco and potato. PVYN isolates can be...

RESUMEN Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT...

Since the concept of pathogen derived resistance (PDR) was proposed in 1985, genetic transformation of plants to express virus-derived sequences has been used to engineer resistance to many viruses. This paper reviews PDR approaches to Potato virus Y (PVY, type member of the genus Potyvirus). PDR to viruses operates often through RNA-mediated resistance mechanisms that do not require protein ex...

A full-length cDNA clone of the potato virus Y (PVY) genome was obtained after cloning difficulties in Escherichia coli were overcome. These difficulties were mainly due to the expression of the CI gene from upstream prokaryotic promoter-like elements within the PVY genome. To overcome this problem, PVY cDNA was maintained in two subclones which were ligated before infection. A plasmid in which...

Molecular evolution of RNA viruses: Plant RNA viruses evolve more rapidly than the DNA of their plant hosts due to a higher mutation rate. Mutational processes include nucleotide substitution that replaces one nucleotide by another, and insertions and/or deletions that result in sequence length changes. In addition to mutation, inter-strain recombination can produce new mosaic strains9. It is i...