Viruses infecting wild flora may have a significant negative impact on nearby crops, and vice-versa. Only limited information is available on wild species able to host economically important viruses that infect sweetpotatoes (Ipomoea batatas). In this study, Sweet potato chlorotic fleck virus (SPCFV; Carlavirus, Betaflexiviridae) and Sweet potato chlorotic stunt virus (SPCSV; Crinivirus, Closte...

background: the advent of recombinant dna technology has facilitated heterologous expression of proteins from various sources in different host systems including escherichia coli. if a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

Of seven plant viruses tested, all except tobacco mosaic showed the phenomenon of photoreactivation, i.e. plants exposed to visible light after inoculation with preparations partially inactivated by ultraviolet radiation produced more local lesions than plants kept in darkness. Five straiIis of tobacco mosaic virus, which differed widely in their pathogenicity and other properties, were tested,...

Sweet potato chlorotic stunt virus (genus Crinivirus) belongs to the family Closteroviridae, members of which have a conserved overall genomic organization but are variable in gene content. In the bipartite criniviruses, heterogeneity is pronounced in the 3'-proximal region of RNA1, which in sweet potato chlorotic stuat virus (SPCSV) encodes two novel proteins, RNase3 (RNase III endonuclease) a...

The cucumber mosaic virus (CMV) 2b viral suppressor of RNA silencing (VSR) inhibits host responses to jasmonic acid (JA), a chemical signal regulating resistance to insects. Previous experiments with a CMV subgroup IA strain and its 2b gene deletion mutant suggested that VSRs might neutralize aphid (Myzus persicae) resistance by inhibiting JA-regulated gene expression. To further investigate th...

Sweet potato belongs to Ipomoea series batatas and is thought to have originated in Central America and Northern South America. World sweet potato production is around 106 million tons in an area of about 8.1 million ha. Asia is the world’s largest sweet potato-producing region and China is the world’s leading sweet potato-producing country accounting for about 76% of production. Sweet potato h...

We compared infection of Nicotiana benthamiana plants by the positive-sense RNA viruses Cucumber mosaic virus (CMV), Potato virus Y (PVY), and by a Potato virus X (PVX) vector, the latter either unaltered or expressing the CMV 2b protein or the PVY HCPro suppressors of silencing, at 25°C vs. 30°C, or at standard (~401 parts per million, ppm) vs. elevated (970 ppm) CO2 levels. We also assessed t...

Sweetpotato (Ipomoea batatas (L.) Lam.) is the third most important root crop in the world, ranking seventh among all crops in global production and fourth in developing countries after rice, wheat, and corn (10). In Africa, sweetpotato plays a vital role in peoples’ ability to sustain their families, especially during periods of hardship. However, sweetpotato plants, like other crops, are sign...