Boar spermatozoa were prepared for intracytoplasmic sperm injection (ICSI) by two different treatments to facilitate sperm chromatin decondensation and improve fertilisation rates after ICSI in pigs: spermatozoa were either frozen and thawed without cryoprotectants, or treated with progesterone. Morphological changes of the sperm heads after the treatments were examined and then the activation ...

A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acros...

This study aimed to compare post-thaw quality of boar semen following freezing in an ostrich egg yolk lipoprotein (LPFo) extender supplemented with 0%, 0.25% and 0.50% Orvus Es Paste (OEP). Sperm assessments included total motility (TMOT), mitochondrial function (MF), plasma membrane integrity (PMI) and acrosome integrity (normal apical ridge, NAR). Considerable variations among boars and OEP t...

Sapera goat is a dairy resulting from cross between Saanen buck and an Ettawah doe. These small ruminants are reared by rural households for poverty reduction undernutrition prevention. Breeding of through artificial insemination (AI) technique was expected to accelerate the increase in population. Unfortunately, frozen semen not available yet. This study aims determine effect distance straw li...

abstract background: sperm parameters and motion kinetics are affected by cryopreservation. objective: the main purpose of the current study was to determine the effect of different concentrations of trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. materials and methods: semen was collected from 20 normal donors and divided into five aliquots prior to c...


 This study aimed to determine the effect of glycerol concentration on motility, viability and abnormalities frozen thawed spermatozoa Bali cattle. The was conducted using a completely randomized design with 5 treatments 6 replications. consisted T0 (0% glycerol), T1 (6% T2 (12% T3 (18% P4 (24% glycerol). results showed that administration had very significant (p<0.01) viability, abnor...

HARRIS et al.1), observing ultrastructural changes in rapidly frozen fowl spermatozoa, noted destructive effects on the acrosome, the midpiece, and the cytoplasmic membrane. Similar ultrastructural damages were seen by BAKST and SEXTON2) in frozen-thawed fowl and turkey spermatozoa under transmission and scanning electron microscopes. However, they gave little information on the possible mechan...

In mares, a physiological endometritis ensues following the deposition of semen into the uterus. This response is thought to be due to the antigenic nature of spermatozoa, specifically the activation of complement, and may be modulated by the presence of seminal plasma component of the semen. The removal of seminal plasma during cryopreservation of spermatozoa may account for the longer duratio...

A comparison of spermatozoa from intact boars and from boars without seminal vesicles, frozen in various diluents, was made to determine any differences in sperm quality after thawing and the conception rates of gilts. Sperm samples were frozen at -196 degrees C. 50 semen pellets were thawed after 1-3 weeks storage and assessed for postthaw motility at 37 degrees C. The percentage of eosinop...