نتایج جستجو برای: pore system

تعداد نتایج: 2266274  

2015
Tobias Stuwe Ana R. Correia Daniel H. Lin Marcin Paduch Vincent T. Lu Anthony A. Kossiakoff André Hoelz

2014
Min Xue Jeffrey I. Zink

The microenvironment inside of the pores of mesoporous silica nanoparticles is probed using spectroscopic techniques. The probe molecules are sealed inside of the pores by a nanovalve system that is capable of controlling the access to the pore and ensuring the exclusive probing of the pore environment without any interference from the probe molecules on the outer surface of the particles or fr...

Journal: :Biophysical journal 2003
Mark Bates Michael Burns Amit Meller

The dynamics of single-stranded DNA in an alpha-Hemolysin protein pore was studied at the single-molecule level. The escape time for DNA molecules initially drawn into the pore was measured in the absence of an externally applied electric field. These measurements revealed two well-separated timescales, one of which is surprisingly long (on the order of milliseconds). We characterized the long ...

Journal: :Physical review. E, Statistical, nonlinear, and soft matter physics 2008
K E Washburn C H Arns P T Callaghan

We use the propagator-resolved transverse relaxation exchange experiment to characterize the pore space and fluid behavior of water saturated, tight-packed quartz sand. The experiment uses T2 exchange plots to observe the number of molecules that shift their environment for a range of mixing times. The propagator dimension allows us to determine how far the molecules have moved. The peak intens...

Journal: :Trends in cell biology 1994
M P Rout S R Wente

Nuclear pore complexes (NPCs) are enormous macromolecular structures that mediate the active exchange of proteins and RNPs between the nucleus and cytoplasm. Recent work has resulted in a windfall of identified NPC polypeptides, many with unique sequences. Several of the proteins have been shown to be part of extended cytoplasmic and nucleoplasmic NPC filaments. Biochemical, structural and gene...

2015
Rebecca L. Adams Laura J. Terry Susan R. Wente

FG nucleoporins (Nups) are the class of proteins that both generate the permeability barrier and mediate selective transport through the nuclear pore complex (NPC). The FG Nup family has 11 members in Saccharomyces cerevisiae, and the study of mutants lacking different FG domains has been instrumental in testing transport models. To continue analyzing the distinct functional roles of FG Nups in...

Journal: :Scientific reports 2016
Nikola Kellner Johannes Schwarz Miriam Sturm Javier Fernandez-Martinez Sabine Griesel Wenzhu Zhang Brian T Chait Michael P Rout Ulrich Kück Ed Hurt

We describe a method to genetically manipulate Chaetomium thermophilum, a eukaryotic thermophile, along with various biochemical applications. The transformation method depends on a thermostable endogenous selection marker operating at high temperatures combined with chromosomal integration of target genes. Our technique allows exploiting eukaryotic thermophiles as source for purifying thermost...

Journal: :Biochemical Society transactions 2010
Jindriska Fiserova Martin W Goldberg

Eukaryotic cells have developed a series of highly controlled processes of transport between the nucleus and cytoplasm. The present review focuses on the latest advances in our understanding of nucleocytoplasmic exchange of molecules in yeast, a widely studied model organism in the field. It concentrates on the role of individual proteins such as nucleoporins and karyopherins in the translocati...

2015
Akira HASHIMOTO-GOTOH Takahiro MATSUKI Takayuki MIYAZAWA

Here, we report a first study of virus removal by a novel membrane filtration system, named the “Pore Diffusion”. The “Pore Diffusion” manipulated the direction of circulating flow from vertical to parallel to the membrane, thereby achieved to alter the transmembrane pressure as low as possible. We compared the viral activity between before and after filtration by both infectivity assay and rea...

2013
Andreas F.-P. Sonnen Philipp Henneke

Protein toxins are important virulence factors contributing to neonatal sepsis. The major pathogens of neonatal sepsis, group B Streptococci, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus, secrete toxins of different molecular nature, which are key for defining the disease. Amongst these toxins are pore-forming exotoxins that are expressed as soluble monomers prior to enga...

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