Surface antigens of Actinomyces viscosus T14V were released from cell walls by digestion with lysozyme. These were separated by ion-exchange and gel filtration chromatography into fractions rich in carbohydrate or protein. The former contained a polysaccharide high in 6-deoxytalose, along with a peptide fragment from the cell wall. In the protein-rich fractions, material of high molecular weigh...

Wax D fraction extracted from human tubercle bacilli is a macromolecular peptide-glycolipid consisting of mycolic acid, polysaccharide, and peptide. Wax D fraction obtained from human tubercle bacilli showed characteristic biological activities such as adjuvant activity in the immune response, the induction of adjuvant arthritis in rats, and growth factor activity for pathogenic Leptospira when...

The effects of Cordyceps sinensis (CS) and its extracted fractions on steroidogenesis in MA-10 cells were determined. Different concentrations of CS and 3 fractions of CS (F1, a water-soluble polysaccharide; F2, a water-soluble protein; and F3, a poorly water-soluble polysaccharide and protein) were added to MA-10 mouse Leydig tumor cells with or without human chorionic gonadotropin (hCG), and ...

1. Protein fractions derived from tubercle bacilli are toxic to the interior of Amoeba dubia but have no action on the plasmalemma. 2. Phosphatide fractions dissolve the plasmalemma but have no effect on the internal cytoplasm. 3. The fatty acid fraction has a marked solvent action on the plasmalemma when brought in contact with the surface of the cell. When injected it may slowly penetrate the...

BACKGROUND AND AIMS Covalent linkages between xyloglucan and rhamnogalacturonan-I (RG-I) have been reported in the primary cell walls of cultured Rosa cells and may contribute to wall architecture. This study investigated whether this chemical feature is general to angiosperms or whether Rosa is unusual. * METHODS Xyloglucan was alkali-extracted from the walls of l-[1-3H]arabinose-fed suspens...

Porcine pancreatic RNase was isolated from the pancreatic secretion of animals with permanent fistulas by a combination of DEAE-cellulose chromatography, adsorption-elution from sulfoethyl-Sephadex, and gel filtration on Sephadex G-100. On the basis of these experiments, a practicable, large scale procedure was developed for the preparation of the enzyme in quantities of 100 to 200 mg. Porcine ...

Neutral and acidic polysaccharides and their protein complexes were fractionated and purified from the brown seaweed umitoranoo (Sargassum thunbergii) by fractional extraction, iron-exchange chromatography, and gel filtration. Thirty-one polysaccharide fractions were obtained and tested for antitumor activity in mice with Ehrlich carcinoma transplanted i.p. Two of the fractions, GIV-A ([alpha]2...