Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variabl...

Random-amplified-polymorphic-DNA(RAPD) was assayed to detect the genetic variation of 6 barley generations from Iraq. Four primers generated a total 17 scoreable bands in RAPD analysis) and resolving power, three polymorphic differed (Rp). The use marker systems distance among generation discovered be beneficial. dendrograms indicate diverse grouping barely specimens, although we did see that c...

the genetic diversity of 20 iranian populations of bunium persicum has been evaluated with random amplified polymorphic dna (rapd) and amplified fragment length polymorphism (aflp) markers. fresh leaves of seedlings from each population were used for genomic dna extraction. analysis of banding patterns of 15 rapd primers and 17 aflp primer combinations, revealed 192 (86%) and 228 (75%) polymorp...

UNLABELLED PREMISE OF THE STUDY Microsatellite markers from the genome of Diplarche multiflora were developed and characterized to investigate its genetic diversity and population structure. • METHODS AND RESULTS Twenty-seven microsatellite loci were isolated from the genome of D. multiflora using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Of these marker...

A total of nine microsatellite loci were isolated and characterized in the Chilean kelp Lessonia nigrescens Bory. Using two different enriched libraries, we observed 1-14 alleles per locus in two samples of 21 kelp individuals each. The observed heterozygosities ranged from 0.05 to 0.80 and all loci are in Hardy-Weinberg equilibrium for one or both samples. Seventeen samples collected from diff...

Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...

The remarkable recent advances in molecular genetics are grounds for considerable optimism in psychiatry, where many diseases appear to have an hereditary component. Most notably, the discovery of restriction fragment-length polymorphisms (RFLPs) has greatly increased the number of markers available for linkage studies. Linkage analysis has two basic requirements. First, co-operative families m...

PREMISE OF THE STUDY Microsatellite markers were developed for investigating the population dynamics of Myriopteris lindheimeri (Pteridaceae), an apomictic triploid fern endemic to deserts of the southwestern United States and Mexico. METHODS AND RESULTS Using 454 sequencing, 21 microsatellite markers were developed. Of these, 14 were polymorphic with up to five alleles per locus and eight ma...