The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics o...

canine distemper virus (cdv) is the cause of canine distemper (cd) which is a severe and highly contagious disease in dogs. in the present study, a duplex reverse transcription polymerase chain reaction (rt-pcr) method was developed for the detection and differentiation of wild-type and vaccine strains of cdv. four primers were designed to detect and discriminate the two viruses by generating 6...

The reverse transcription polymerase chain reaction (RT-PCR), reverse passive haemagglutination assay (RPHA) and agar gel precipitation test (AGPT) were compared for the detection of infectious bursal disease virus (IBDV) in various organs of experimentally infected broilers. RT-PCR and RPHA required a time of 8 and 1 hour, respectively, whereas AGPT showed precipitation reaction at least after...

Cherax reovirus infects redclaw crayfish (Cherax quadricarinatus) and it may be involved in mortalities between 5-20 % stunting of up to 40 survivors. The sequence the RNA-dependent RNA polymerase was used develop a reverse transcription, quantitative, PCR (RT-qPCR) which specific against seven other crustacean viruses (Athtab bunyavirus, Chequa iflavirus, Macrobrachium rosenbergii nodavirus, Gil...

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

The human immunodeficiency virus type 1 (HIV-1) develops resistance to all available drugs, including the nucleoside analog reverse transcriptase inhibitors (NRTIs) such as AZT. ATP-mediated excision underlies the most common form of HIV-1 resistance to AZT. However, clinical data suggest that when HIV-2 is challenged with AZT, it usually accumulates resistance mutations that cause AZT resistan...

Abstract A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and being used for screening procedure all incoming passengers to Northern Cyprus SARS-CoV-2. This study investigated compatibility of two different RT-qPCR methodologies Diagnovital® Bio-Speedy® by re-analyzing previously confirmed positive samples. total 43 samp...

Coronavirus disease 2019 (COVID-19) has impacted people of all ages, including neonates. However, confirmed data on its vertical transmission is still scarce. As a result, displaying various cases COVID-19 in neonates may assist understanding the virus's behavior during pregnancy. This report shows 32-year-old mother with 5-day history acute respiratory tested positive for by real-time polymera...

To assess the performance of second reading chest compute tomography (CT) examinations by expert radiologists in patients with discordance between reverse transcription real-time fluorescence polymerase chain reaction (RT-PCR) test for COVID-19 viral pneumonia and CT report. Three hundred seventy-eight were included this retrospective study (121 women 257 men; 71 years median age, a range 29–93...

BACKGROUND This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic approaches on blood samples from patients with esophageal squamous cell cancer. MATERIALS AND METHODS Preoperative blood samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase chain reaction (RT-PCR)...