نتایج جستجو برای: polyacrylamide gels
تعداد نتایج: 31220 فیلتر نتایج به سال:
Silver staining of nucleic acid has been widely used in molecular marker analysis such as simple sequence repeat (SSR), single-strand conformation polymorphism (SSCP), and amplified fragment-length polymorphism (AFLP). Many alternatives to silver staining methods have been described, but these methods are not efficient or cost-effective. Here we report a silver staining method that requires les...
Histidine decarboxylase was purified 800-fold from the kidneys of thyroxine-treated mice. The purification procedure included precipitation of protein from a crude supernatant after heating it to 55 degrees C at pH 5.5, fractionation with (NH4)2SO4, phosphocellulose column chromatography, chromatofocusing, DEAE-Sepharose column chromatography, gel filtration on Sephacryl S-300 and preparative p...
The variability in esterases of the entomogenous fungus Metarhizium anisopliae was determined electrophoretically on 8.5% polyacrylamide gel. Ten isolates from diverse taxonomic groups of insects were analyzed. The electrophoretic analysis showed differences and similarities between these isolates and it was possible to distinguish six different patterns. The results obtained show a great polym...
5.8 S RNA-protein complexes were prepared using purified yeast 5.8 S RNA and proteins from the large ribosomal subunit of rat liver. Formation of such hybrid complexes, as measured by Millipore filtration, was dependent on protein concentration. Binding of proteins to the RNA could approach saturation. Such complexes were isolated from sucrose density gradient centrifugation and shown to contai...
chain electrophoresis: a new approach to the determination of the GgAg ratio in fetal haemoglobin and to studies of globin synthesis. Br. J. Haematol. 44:527-534. 2.Dionisi, H.M., S.K. Checa and A.M. Viale. 1995. Protein immunoblotting of stained gels. BioTechniques 19:348-349. 3.Harrington, M.G. 1990. Elution of proteins from gels, p. 488-495. In M.P. Deutscher (Ed.), Methods in Enzymology, Vo...
Thin layers of gels with mechanical properties mimicking animal tissues are widely used to study the rigidity sensing of adherent animal cells and to measure forces applied by cells to their substrate with traction force microscopy. The gels are usually based on polyacrylamide and their elastic modulus is measured with an atomic force microscope (AFM). Here we present a simple microfluidic devi...
Pot experiments showed that Rhizobium japonicum cells entrapped in a polyacrylamide gel could be used as an inoculant for soybeans and compared favorably to laboratory-made peat base inoculant containing the same bacterial strain.
A procedure for high resolution electrophoresis of isozymes of the mosquito Aedes aegypti using nonlinear (2.5%-20%) polyacrylamide gradient gel slabs is described. Crude mosquito homogenates were electrophoresed on gradient and on homogeneous (7%) polyacrylamide gels and stained for esterase (EST), isocitrate dehydrogenase (IDH) and malate dehydrogenase (MDH). The resulting zymograms were comp...
In this work, nanocomposite (NC) hydrogels based on polyacrylamide/chromium triacetate were prepared at different reaction mixture (gelant) volumes and their crosslinking process and viscoelastic behaviors were studied. The X-ray diffraction (XRD) patterns taken from the NC hydrogels containing laponite nanoparticles did not show any distinct characteristic basal reflection for all of the NC hy...
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