The equilibrium adsorption of bovine serum albumin (BSA) on poly-3-hydroxybutyrate (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) surfaces was studied at different solution concentrations. data were fitted to the Langmuir Freundlich models. protein behaviour further investigated by analysing conformation surface adsorbed BSA using Fourier transform infrared (FTIR) spectroscopy wi...

The relationship between butirosin biosynthesis and certain biochemical characteristics related to sporulation in a strain of Bacillus circulans NRRL B-3313 was examined. The cellular content of dipicolinic acid increased while the amount of poly-beta-hydroxybutyrate decreased with changes in antibiotic productivity. Oligosporogenous mutants failed to synthesize the antibiotic and to degrade po...

β-Hydroxybutyrate, a ketone body that is used as an energy source in organs such as the brain, muscle, and heart when blood glucose is low, is produced by fatty acid oxidation in the liver under the fasting state. Endoplasmic reticulum (ER) stress is linked with the generation of intracellular reactive oxygen species and the accumulation of misfolded protein in the ER. ER stress is known to ind...

Adipocytes are the most abundant stromal partners in breast tissue. However, the crosstalk between breast cancer cells and adipocytes has been given less attention compared to cancer-associated fibroblasts. Here we find, through systematic screening, that primary mammary gland-derived adipocytes (MGDAs) promote growth of breast cancer cells that express monocarboxylate transporter 2 (MCT2) both...

BACKGROUND Ketone bodies are known to substitute for glucose as brain fuel when glucose availability is low. Ketogenic diets have been described as neuroprotective. Similar data have been reported for triheptanoin, a fatty oil and anaplerotic compound. In this study, we monitored the changes of energy metabolites in liver, blood, and brain after transient brain ischemia to test for ketone body ...

Biodegradable plastics has gained significant commercial interest in recent years as they are having similar properties to that of the synthetic plastic used now a days, they are completely bio-degradable, are produced from natural (microbial) sources and are biocompatible in human system. These properties make PHB as an ideal candidate for various applications in medical field. The present art...

Batch culture of Ralstonia eutropha using the recycled gas closed circuit culture system was conducted in order to develop a practical fermentation system for industrial autotrophic culture for poly (hydroxybutrate) production. The gas phase of the culture system consisted of substrate gas so that gases in this culture could be recycled as long as the amount of the gas consumed would be repleni...

The use of detailed models for bioprocess design and control has been limited as accurate estimation of model parameters is often difficult. In this paper, the parameter estimation problem for a mechanistic model of the production of a biopolymer, poly-β-hydroxybutyrate (PHB), is examined in detail. Parameter estimation was undertaken using previously published data. Three parameter sets were o...