Aggregation of human platelets by bovine plasma was recently recognized as a marker for the study of the antihemophilic and von Willebrand factors. A similar marker in porcine plasma is shown to be specific for the platelet-active von Willebrand factor of plasma, but not for the antihemophilic factor (factor VIII). A new quantitative assay for the von Willebrand factor is based on the dose-resp...

Ethanol is known to inhibit platelet aggregation. In order to examine effects of ethanol on platelet aggregation, isolated platelet-rich plasma and platelet suspension are often used. However, it remains to be clarified whether and how ethanol affects platelet aggregation in whole blood. In this concise study, we examined effects of ethanol on platelet aggregation induced by different stimulant...

The purpose of this study was to evaluate in vitro the effects of hydrocortisone and aminophylline on adenosine diphosphate (ADP)-induced platelet aggregation in horses. Blood samples from 30 healthy Thoroughbred horses were collected by via jugular venipuncture to assess platelet aggregation. Platelet-rich and platelet-poor plasma were prepared from all samples by centrifugation and divided in...

Previous observations that platelet guanosine 3’. 5’-monophosphate (cyclic GMP) accumulation occurred during platelet aggregation and also in the presence of nitroprusside. which inhibits aggregation. prompted an evaluation of the effects of nitroprusside. nitric oxide. and related agents on human platelet aggregation. Adenosine diphosphate (ADP) was employed to induce irreversible aggregation ...

BACKGROUND Platelet dysfunction is a well-established disturbance in haemodialysis (HD) patients. Propyl gallate is a synthetic platelet activator with the property to stimulate platelet aggregation. The aim of the present study was to evaluate the influence of a single haemodialysis session on propyl gallate-induced platelet aggregation. METHODS Thirty-nine HD patients were enrolled in the s...

In this study we have examined the mechanism of platelet aggregation under physiological flow conditions using an in vitro flow-based platelet aggregation assay and an in vivo rat thrombosis model. Our studies demonstrate an unexpected complexity to the platelet aggregation process in which platelets in flowing blood continuously tether, translocate, and/or detach from the luminal surface of a ...

Staphylococcus aureus can stimulate activation and aggregation of platelets, which are thought to be factors in the development of infective endocarditis. Previous studies have identified clumping factor A (ClfA) and fibronectin binding proteins A and B (FnBPA and FnBPB) as potent platelet aggregators. These proteins are able to stimulate rapid platelet aggregation by either a fibrinogen- or a ...

Genetic variation in the platelet endothelial aggregation receptor 1 (PEAR1) gene, most notably rs12041331, is implicated in altered on-aspirin platelet aggregation and increased cardiovascular event risk. We prospectively tested the effects of aspirin administration at commonly prescribed doses (81, 162, and 324 mg/day) on agonist-induced platelet aggregation by rs12041331 genotype in 67 healt...

BACKGROUND The study of platelet aggregation is essential to assess in vitro platelet function by different platelet activation pathways. OBJECTIVE To assess aggregation and biochemical parameters of random platelet concentrates produced at the Fundação HEMOAM using the quality control tests defined by law. METHODS Whole blood samples from 80 donors and the respective platelet concentrate u...

BACKGROUND Platelet aggregation during aspirin treatment displays considerable inter-individual variability. A genetic etiology likely exists, but it remains unclear to what extent genetic polymorphisms determine platelet aggregation in aspirin-treated individuals. AIM To identify platelet-related single nucleotide polymorphisms (SNPs) influencing platelet aggregation during aspirin treatment...