background and objective: organisms producing ctx-m β-lactamases are emerging as a source of resistance to oxyiminocefalosporins such as ceftriaxone and ceftazidime. however, the laboratory detection of these strains is not well defined. in this study, phenotypic assay for screening of extended-spectrum β-lactamases producing strains and molecular assay for the identification of ctx-m β-lactama...

background & objectives:extended-spectrum-b-lactamase (esbl)-producing strains of klebsiella pneumoniaare an important cause of many serious infections in hospitalized and nonhospitalized patients and delayed treatment of these infections in crease chance of death in patients. this study was performed to determine the prevalence of esbl-producing k. pneumonia and to evaluate the frequency o...

The study included isolation and diagnosis of Escherichia coli from clinical animal sources (humans, cows, sheep, birds, chickens, fish, gecko). It collected 275 samples distributed to 125 taken urine 150 other organisms with 25 per organism. isolates used in the were diagnosed on appearance, microscopy, chemical tests, as well confirmatory testing isolations through use Api 20 E tape, where 41...

Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (pAmpC) are enzymes able to hydrolyze a large variety of β-lactam antibiotics, including third-generation cephalosporins and monobactams. Broilers and broiler meat products can be highly contaminated with ESBL- and pAmpC-producing Escherichia coli strains, also known as extended-spectrum cephalosporin (ESC)-resistant ...

The spread of antibiotic resistant bacteria worldwide presents a major health threat to human health care that results in therapy failure and increasing costs. The transfer of resistance conferring plasmids by conjugation is a major route by which resistance genes disseminate at the intra- and interspecies level. High similarities between resistance genes identified in foodborne and hospital-ac...

New Delhi metallo-β-lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem-resistant Escherichia coli clinical strain WCHEC13-8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 μg/ml) and meropenem (MIC, 128 μg/ml) and belonged to ST3835. bla NDM-1 was the only ...