Browning is one of the severe problems in plant tissue culture that hampers successful vitro propagation plants especially woody and perennial plants. In order to control browning problem, different efforts has been made such as presoaking explants antioxidant solution, incorporation antioxidants into medium, culturing dark period frequent subculturing explants. Presoaking solutions like polyvi...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

Haberlandt envisioned the concept of plant tissue culture and provided the basic work for the cultivation of plant cells, tissues and organs culture. In recent years, a number of plants has been investigated and reported possessing medicinal values. Over-utilization and continuous depletion of traditional medicinal plants have affected their supply and loss of genetic biodiversity. Many Endange...

Background & Aim: Investigating the possibility of using medicinal plant’s seeds substitute for agar gel in plant tissue culture and determining the optimal practical conditions of them is the aim of this study. Experimental: In order to study the new gelling agents for using in plant tissue culture, we designed a tow part experiment by means of the seeds of medicinal plants such as Linum ...

Background & Aim: Investigating the possibility of using medicinal plant’s seeds substitute for agar gel in plant tissue culture and determining the optimal practical conditions of them is the aim of this study. Experimental: In order to study the new gelling agents for using in plant tissue culture, we designed a tow part experiment by means of the seeds of medicinal plants such as Linum ...