Mycobacterium tuberculosis 6-kDa early secretory antigenic target (ESAT-6) is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand (FL) that induces potent immune response has been used as an adjuvant in vaccine development. In this study, a new recombinant plasmid (pIRES-epitope-peptides-FL) encoding three T cell epitope...

In study 1 (n = 51, M age = 21.4 years, SD = 5.7), the validity of the Portuguese adaptation of the Gudjonsson Suggestibility Scales (Pires, 2011) was shown through the comparison of means of the original (Gudjonsson, 1997) and the translated scales and the analysis of the correlations between the GSS1 and GSS2 scores. The relationships between interrogative suggestibility and the big five were...

Cardiomyocytes express mRNA for all major subunits of ATP-sensitive potassium (K(ATP)) channels: KIR6.1, KIR6.2, SUR1A, SUR2A, and SUR2B. It has remained controversial as to whether KIR6.1 may associate with KIR6.2 to form the tetrameric pore of K(ATP) channels in cardiomyocytes. To explore this possibility, cultured rat cardiomyocytes were examined for an inhibition of K(ATP) current by overex...

Uniform patterning of cells is highly desirable for most cellular studies involving cell-cell interactions but is often difficult in an in vitro environment. This paper presents the development of a collagen-coated planar interdigitated ring electrode (PIRE) array utilizing positive dielectrophoresis to pattern cells uniformly. Key features of the PIRE design include: (1) maximizing length alon...

Millions of people worldwide are currently infected with human papillomavirus (HPV), herpes simplex virus (HSV) or human immunodeficiency virus (HIV). For this enormous contingent of people, the search for preventive and therapeutic immunological approaches represents a hope for the eradication of latent infection and/or virus-associated cancer. To date, attempts to develop vaccines against the...

Maria Teresa Godinho, CIO-Dep. de Matemática, Instituto Politécnico de Beja, [email protected] Luis Gouveia, CIO-DEIO, Faculdade de Ciências da Universidade de Lisboa, [email protected] Thomas L. Magnanti, Dep. of Electrical Engineering and Computer Science and Sloan School of Management, MIT, Cambridge, [email protected] Pierre Pesneau, Université de Bordeaux 1, Institut IMB, France, P...

Screening of unusual forms of diabetes might not have been accounted by the Brazilian public health system Ricardo Emidio Navarrete de Toledo, Wimbler Pires Silva, Laura Priscila N Toledo, Maryane Caroline de Toledo, Rebeca Esteves Matos Rodrigues Ramiro, Bruna Maria Grosso Mascarenhas, Adriana Seixas Costalonga, Julio Cesar Rebelo Sampaio Filho, Ana Julia Garcia Pereira, Andrea Abbud, Giovanna...

introduction: long term complications of hepatitis c virus (hcv) infection include fibrosis, cirrhosis, and hepatocellular carcinoma and although the disease is treatable with the newly introduced direct acting antivirals, but factors such as drug resistance, viral genotype and adverse effects can limit the effectiveness of therapy. therefore, development of effective, safe and affordable proph...

In this article, motivated by the study of symplectic structures on manifolds with boundary and systematic b $b$ -symplectic started in Guillemin, Miranda, Pires Adv. Math. 264 (2014), 864–896, we prove a slice theorem for Lie group actions manifolds.

Whole-body optical imaging is an external and noninvasive procedure that enables the continuous visual monitoring of malignant growth and spread within intact animals. The human colon adenocarcinoma cell-line HCT-15 was transfected with a pIRES 2-EGFP vector, and stable enhanced green fluorescence protein (EGFP) expression was established (Fig.1). Approximately 10(6) HCT-15 EGFP stable transfec...