Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals and reduces phosphorus pollution of animal waste. Our objectives were to express an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae and to determine the effects of glycosylation on the phytase's activity and thermostability. A 1.4-kb DNA fragment containing the coding region of the ...

BACKGROUND: Phosphorous is one of the expensive nutrients in poultry feed. Therefore, improving the bioavailability of this nutrient in feed ingredients could be effective for lowering the cost of feed. OBJECTIVE: This study was performed to evaluate the effect of pre-treatment of feed ingredients by commercial enzymes and different levels of pH on releasing of phosphorus from phytate under in ...

Plant seeds that have high phytate content are used as animal feed. Phytases, enzymes that catalyze the breakdown of phytate into inorganic phosphorus and myoinositol phosphate derivatives, have been intensively studied in recent years and gained immense attention because of their application in reducing phytate content in animal feed and food for human consumption, thus indirectly lowering env...

Previous reviews have covered our work on the efficacy of a new E. coli-derived phytase (expressed in yeast) for releasing phytate-bound phosphorus (P) present in corn-soybean meal diets for young pigs, growing-finishing pigs, broiler chicks and laying hens (Baker and Augspurger, 2002; Augspurger, 2003; Augspurger et al., 2003a). It is not our intent to repeat this information, but instead to p...

113 Effects of dietary non-phytate phosphorus level and phy­ tase on growth performance of Cherry Valley duck. Z. Z. Ren*1, Q. F. Zeng1, X. M. Ding1, S. P. Bai1, Y. H. Luo1, J. P. Wang1, K. Y. Zhang1, and G. Xu2, 1Institute of Animal Nutrition, Animal Nutrition and Feed Engineering, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Sichuan, China, 2Sichuan Chuanheng Chemi­ ca...

Five copper (Cu) sources were studied at pH 2.5, 5.5, and 6.5 to determine how Cu affects phytate phosphorus (PP) hydrolysis by phytase at concentrations up to 500 mg/kg diet (60 min, 40-41 degrees C). Subsequently, Cu solubility with and without sodium phytate was measured. Adding Cu inhibited PP hydrolysis at pH 5.5 and pH 6.5 (P < 0.05). This inhibition was greater with higher concentrations...