نتایج جستجو برای: photoreactivation

تعداد نتایج: 311  

2006
Ronald D. Ley B. Ann Sedita Donald D. Grube Michael Fry

cent sun lamp (280 to 400 nm), respectively. Enzymatic photoreactivation with yeast photoreactivating enzyme showed that -80% of the endonuclease-sensitive sites were cyclobutyl pyrimidine dimers. In both strains of mice the pyrimidine dimers remained in high-molecular-weight DNA for 24 hr after irradiation. These data show that mouse epi thelial cells in vivo have little or no capacity for the...

Journal: :Journal of bacteriology 1997
R Kato K Hasegawa Y Hidaka S Kuramitsu T Hoshino

The photolyase gene from Thermus thermophilus was cloned and sequenced. The characteristic absorption and fluorescence spectra of the purified T. thermophilus photolyase suggested that the protein has flavin adenine dinucleotide as a chromophore. The second chromophore binding site was not conserved in T. thermophilus photolyase. The purified enzyme showed light-dependent photoreactivation acti...

Journal: :Journal of bacteriology 1951
J S BROWN

Kelner (1949a) first reported that mutations to phage resistance induced in Escherichia coli by ultraviolet light could be reversed by immediate irradiation with white light. In addition he noted that end-point mutations in contrast to zero-point mutations were not reversed by visible light (1949b). At the same time Novick and Szilard (1949) reported that light-reactivation of E. coli reduced t...

Journal: :Cancer research 2000
V Chiganças E N Miyaji A R Muotri J de Fátima Jacysyn G P Amarante-Mendes A Yasui C F Menck

Photolyase absorbs blue light and employs the energy to remove UV-induced DNA damage, cyclobutane pyrimidine dimers, or pyrimidine pyrimidone (6-4) lesions. These enzymes have been found in many living organisms ranging from bacteria to aplacental mammals, but their photoreactivation effect, such as survival increase of UV-irradiated cells by light-illumination, has not been identified in place...

Journal: :Nucleic acids research 1998
S Nakajima M Sugiyama S Iwai K Hitomi E Otoshi S T Kim C Z Jiang T Todo A B Britt K Yamamoto

UV radiation induces two major classes of pyrimidine dimers: the pyrimidine [6-4] pyrimidone photoproduct (6-4 product) and the cyclobutane pyrimidine dimer (CPD). Many organisms produce enzymes, termed photolyases, that specifically bind to these damage products and split them via a UV-A/blue light-dependent mechanism, thereby reversing the damage. These photolyases are specific for either CPD...

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