State and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. Complete inactivation of the enzyme was observed after oxidation of 4 histidine residues. From the pH dependence of the photooxidation it becomes evident that the inactivation of the enzyme is not a consequence ...

We describe here a surface-bound, oxide-based procedure for the photooxidation of a family of aromatic hydrocarbons by a phosphate-bearing flavin mononucleotide (FMN) photocatalyst on high surface area metal-oxide films.

Optical rotation measurements and absorption difference spectroscopy of ribonuclease A in different water-acetic acid mixtures showed that at least three conformational situations exist for the protein molecule at different levels of acetic acid concentration. Hematoporphyrin-sensitized photooxidation of the native as well as of the denatured proteins allowed us to detect three discrete states ...

A laser aerosol time-of-flight mass spectrometry (ATOFMS) that can be used for real-time measurement of the size and composition of individual aerosol particles has been designed and manufactured in our laboratory. Particles are introduced into the instrument through a particle beam interface, sized by measuring the delay time between two scattering lasers, and compositionally analyzed using a ...

Different preparative procedures for isolation and purification of plastocyanin were successful to obtain the pure protein from Chlorella vulgaris, Scenedesm us acutus and Spinacia oleracea but not from B um illeriopsis filiform is. Lade of plastocyanin in this alga was further confirmed by the following in comparison with spinach chloroplast material: a. Use of antibodies to block photosynthet...