Beta granules isolated from rat islets of Langerhans and subjected only to phosphotungstic acid had, in negatively stained images, a 50-A periodicity. This periodicity was also observed in thin-section profiles of beta granules in intact cells. In shadowed preparations, the granules were spherical in shape and had irregular edges and surface structure. The presence of such a periodicity in the ...

This relatively simple method for quantitative estimation of lipoprotein X is based on assay of phospholipids in precipitates of lipoprotein X. We first remove other lipoproteins by precipitation with phosphotungstic acid solution. Lipoprotein X is then precipitated from the supernatant fluid with an alkaline solution of magnesium ion, and phospholipid is determined in this precipitate. Results...

This study aimed to determine the causes of bitterness problems in white cheese produced by ultrafiltration (UF). To investigate association between with enzyme use or starter culture, was using UF-pasteurized and UF-raw milk two different coagulating enzymes cultures proteolytic activities. Sensory analysis, protein, water-soluble nitrogen, 12% trichloroacetic acid-soluble 5% phosphotungstic n...

The intermediary metabolism of theophylline has been the subject of a number of studies, but its fate in the body is still unsettled. Kruger and Schmidt (1) working with dogs reported that the administration of theophylline to dogs is followed by the appearance of 3-methylxanthine in the urine. Myers and Wardell (2) and Buchanan, Christman, and Block (3) showed that ingestion of theophylline in...

936 it is known that each fiber has a narrow cortical zone which appears more or less dense (depending on the methods of preparation) than its center . or medulla (Telkka, Fawcett, and Christensen, 1961). Phosphotungstic acid appears to stain the cortex selectively, leaving the medulla unstained, and the demarcation between cortex and medulla is shown to be clearest in the abaxial segment of th...

Serum uric acid levels measured by the phosphotungstic sodium cyanide method have been estimated in 60 patients suffering from a cerebrovascular incident. Patients with diseases known to predispose to hyperuricaemia were excluded. Twenty-five per cent. of patients with acute strokes had hyperuricaemia, and this did not appear to be a transitory phenomenon. These findings suggest that hyperurica...

The reagent is modified as follows: Serum chromogens are destroyed by trisodium phosphate. The use of phosphotungstic acid eliminates the tungstate and sulfate anions, a frequent cause of turbidity. Triethanolamine, added to the carbonate-urea mixture, provides unusual stability of the color through its buffering action. Only 0.2 ml of serum is required. The method is highly reproducible and ac...