A new type of nucleic acid analogues with a phosphoryl guanidine group is described. Oxidation of polymer-supported dinucleoside 2-cyanoethyl phosphite by iodine in the presence of 1,1,3,3-tetramethyl guanidine yields a dinucleotide with an internucleoside tetramethyl phosphoryl guanidine (Tmg) group as the main product. The Tmg group is stable under conditions of solid-phase DNA synthesis and ...

The second-order rate constants for reaction of the Mg2+ complexes of phosphorylated pyridine monoanions with Mg(OH)+ a re 104-106-fold larger than the second-order rate constants for their reaction with water (25 OC, ionic strength 1.5). Of the 106-fold rate enhancement with the phosphorylated 4morpholinopyridine/Mg2 complex, 1 04-fold is attributed to the greater nucleophilicity of Mg(OH)+ co...

Tryptic digestion of the diisopropyl phosphoryl-32P-Scarboxymethyl light chain derivative of human plasmin followed by gel filtration on Sephadex G-75 and G-25 and chromatography on Dowex SOW-X2 has resulted in the isolation of three radioactive peptides. These three peptides, having 31,33, and 34 residues, are all derived from the same segment of the plasmin molecule. Structural determinations...

1. The phosphorylation of milk alkaline phosphatase was studied under various conditions: maximum incorporation occurred at pH5.0 and 50% incorporation at pH6.6-7.0. 2. The phosphorylation was shown to be specific and the results suggest that the active centre of the enzyme is involved in the process. 3. Phosphoryl-enzyme is rapidly hydrolysed at alkaline pH. at pH7.0 the results suggest that a...

As part of a study on enzymic catalysis of group transfer, this laboratory has previously reported on the enzymic transfer of the glucuronyl radical from a variety of donor substrates to a number of acceptor alcohols (1). The phenomenon was studied in detail and conditions for efficient group transfer were investigated. The present paper reports the use of a similar approach in studying the aci...

In the title compound, C(19)H(17)N(2)O(2)P, the six-membered 1,3,2-oxaza-phosphinine ring adopts a boat conformation with the phosphoryl O atom in an equatorial position. The dihedral angle between the 6-methyl-2-pyridyl and phenyl groups is 75.5 (1)°. These substituents are trans to each other, and are oriented at angles of 57.2 (1) and 74.8 (1)°, respectively, to the benzene ring. The crystal...

5-(p-Substituted phenyl)-N-(3-(5-nitrofur-2-yl)allylidene)-1,3,4-thiadiazol-2-amines 10-17 were prepared utilizing different p-substituted benzoic acid through two step reactions. Firstly, thiosemicarbzide was reacted with benzoic acid derivatives 1 in the presence of phosphorus oxychloride to give 2amino-5-substituted-1,3,4-thiadiazoles 2-9. These compounds were reacted with 5-nitro-2-furanacr...

A simple and efficient synthesis of dioxazaphosphinin-2-ones has been developed by the cyclisation of 2-hydroxy-1,2-diphenylethanoneoxime with phosphorus oxychloride in the presence of triethylamine in dry THF to afford the corresponding monochloride. In the next step, subsequent reaction of monochloride with different amines at 50-60 ̊C for 2-3 hours resulted in the formation of the title comp...

In the mol-ecular structure of the title compound, [CaNa(C10H20Cl3N3O2P)3(H2O)], the Ca2+ ion has a slightly distorted octa-hedral coordination environment defined by six O atoms which belong to the carbonyl and phosphoryl groups of the three coordinating ligands. Two Cl atoms of CCl3 groups and four O atoms form the coordination environment of the Na+ ion: three from the carbonyl groups of lig...