Acrosin, a proteolytic enzyme located in the acrosome of sperm, exhibits amidase, esterase, and proteinase activity on synthetic and natural substrates containing arginyl and lysyl residues. Highly purified acrosin preparations from boar acrosomes have endopeptidase activity cleaving only the carboxyl bonds of arginine and lysine with a strong preference for arginine bonds. The Michaelis consta...

In recent reports from this laboratory certain synthetic homioribopolyriucleotides have been found to possess anti-C' 1 activity (1, 2); of these, poly I is the most potent, as little as 0.0076 Mtmole P of this material being capable of inhibiting one C'H0,o (1). Attempts to define the mechanism of action of poly I by use of the R reagents were inconclusive, but suggested that C'1 might be most...

sideration in the literature [13]. As a result, the findings obtained in this investigation may be applicable to the study of PMA in other cell systems. According to Augustinsson [S], each esterase type exists in multiple forms and each animal species has its own typical spectrum of esterases in plasma and tissues [14]. In the presence of marked variations in location, form and activity, one mi...

Acetylcholine (ACh) esterase inhibitors like galantamine and donepezil have been tested as adjunct treatment in schizophrenia. Although ACh esterase inhibition might confer some antipsychotic activity, the role of allosteric potentiation of nicotinic ACh receptors (nAChRs), which is an additional mechanism of galantamine, remains elusive. Therefore, the potential antipsychotic-like effects of g...

Background Angioedema is defined as delimited edema that compromises dermis and subcutaneous. May have different causes hypersensitivity, autoimmune, idiopathic, physical factors, angiotensin-converting enzyme inhibitors and C1 esterase inhibitor consumption or disability. The physiopathologic mechanisms depend on the cause. In addition to detailed history, additional tests are needed for targe...

Following the development of a histochemical method for demonstrating non-specific esterase (1) in tissue sections by enzymatic hydrolysis of Bnaphthyl acetate, the possibility was explored of developing a similar method for lipase together with convenient calorimetric methods for measuring the activity of both enzymes in blood serum. Interest in the enzymes which hydrolyze carboxylic esters st...

this study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (sbp) in cirrhotic patients. forty six children with ascites hospitalized between 2009 and 2010 in children medical center were enrolled in this study. reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

In the course of our studies on enzyme inhibitors produced by microorganisms, two potent esterase inhibitors named ebelactone A and B, were isolated from the cultured broth of the strain MG7-G1 which was closely related to Streptomyces aburaviensis1). They were determined to be new members of mycolic acid /3-lactone. As reported in our previous papers2.3), esterastin, an esterase inhibitor prod...

Sir: We have demonstrated that aminopeptidases, phosphatases and esterases are located not only in cells but also on the cellular membrane of various kinds of animal cells1-3) and that inhibitors of these enzymes modified immune responses4,5). Among these inhibitors, bestatin, amastatin and forphenicine enhanced immune responses, but esterastin which inhibited esterase suppressed immune respons...

The first derivative of carboxypeptidase A, cobalt(III)(ethylenediamine-N,N’-diacetato)(arsanilazotyrosinato 248 (Co(III)(EDDA)(AA-CPA-Zn)), in which only the active site residue tyrosine 248 is blocked, has been shown to be completely peptidase-inactive while retaining esterase activity. Peptides are excellent inhibitors of esterase activity showing Ki values somewhat below the corresponding K...