Arbuscular mycorrhizal fungi (AMF) species and/or phosphorus addition in the soil can affect secondary metabolism production and growth plants. This study evaluated these effects on Piper divaricatum culture, which presents an essential oil rich phenylpropanoids. Growth parameters, leaf volatile composition, total phenolic content, enzymatic activity of lipoxygenase (LOX) phenylalanine ammonia-...

I?henyialanine ammonia-lyase from the yeast Rhodotorula gluiinis was purified by salt fractionations and Sephadex chromatography. Density gradient centrifugation and Sephadex chromatography indicated its molecular weight to be about 275,000. Enzymatic deamination of several ring-substituted phenylalanine analogues and n-phenylalanine was studied. While cinnamic acid, a product of deamination, a...

Biocontrol agents are an environmentally friendly alternative to chemical pesticides against phytopathogens. The effectiveness of metabolites produced by Bacillus atrophaeus B5 for controlling anthracnose Colletotrichum gloeosporioides in harvested fruit and the possible action mechanisms have been studied. Avocado treated with reduced 54% incidence nearly 70% lesion than untreated fruit. treat...

Wounding lettuce (Lactuca sativa L., var. Longifolia) leaves induced an eight-fold increase in the activity of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), and the subsequent accumulation of phenolic compounds and tissue browning at 10 C. PAL is a key enzyme in the synthesis of phenolic compounds. A PAL cDNA was previously isolated by reverse-transcription PCR using total RNA from wounded let...

Enzyme substitution therapy with the phenylalanine ammonia lyase (PAL) is a new approach to the treatment of patients with phenylketonuria (PKU). This enzyme is responsible for the conversion of phenylalanine to trans-cinnamic acid. We assessed the PAL enzyme of the endemic plant Cyathobasis fruticulosa (Bunge) Aellen. for its possible role in the dietary treatment of PKU. The enzyme was found ...

Yeast phenylalanine ammonia-lyase (EC 4.3.1.5) catalyzes the deamination of L-phenylalanine to form trans-cinnamic acid and tyrosine to trans-coumaric acid. Maximal enzyme activity in Rhodotorula glutinis (2 units/g, wet weight, of yeast) was induced in late-log phase (12 to 14 hours) of growth in a culture medium containing 1.0% malt extract, 0.1% yeast extract, and 0.1% L-phenylalanine. A hig...

This work evaluated the effect of drought stress on seedling growth, protein, soluble sugars, and phenolic compounds of two cultivars of canola (RGS003 and Sarigol). Drought stress was induced with polyethylene glycol (PEG) at 0, 5, 10, and 15%. Drought stress increased root fresh weight in both cultivars and the effect of drought was more pronounced in RGS003. Shoot fresh weight reduced in Sar...

Phenylketonuria (PKU), a Mendelian autosomal recessive phenotype (OMIM 261600), is an inborn error of metabolism causing impaired postnatal cognitive development in the absence of treatment. We used the Pah(enu2/enu2) PKU mouse model to study oral enzyme substitution therapy with various chemically modified formulations of phenylalanine ammonia lyase (Av-p.C503S/p.C565S/p.F18A PAL). In vivo stu...