lignin is one of the major characteristics of plant secondary cell wall that provides structural rigidity for the cells and tissues and hydrophobicity to tracheary elements. internode tissues of aeluropus littoralis as a halophyte grass were sampled at different developmental stages (from the first to the fifth internodes ) and under different nacl concentrations. the influences of nacl and int...

in this study, trichoderma harzianum bi was evaluated for its capability to reduce the incidence and severity of the root-knot nematode, meloidogyne javanica, and fusarium wilt, f. oxysporum f. sp. lycopersici, as causal agents of a complex disease of tomato in the laboratory and greenhouse conditions. initial in vitro studies revealed that the parasitism of m. javanica eggs by t. harzianum bi ...

This study was conducted to investigate certain characteristics of the purified Phenylalanine ammonia lyase (PAL). The results revealed that molecular weight 180 kDa by gel filtration and Native-PAGE. optimal pH temperature for enzyme activity were 7 40 °C respectively. stable against values range 6-8. retained 79% its total after incubation one hour at 50 7% (lost 93%) 70 °C. entire completely...

1. Phenylalanine ammonia-lyase (EC 4.3.1.5) was purified to homogeneity from the acetone-dried powders of the mycelial felts of the plant pathogenic fungus Rhizoctonia solani. 2. A useful modification in protamine sulphate treatment to get substantial purification of the enzyme in a single step is described. 3. The purified enzyme shows bisubstrate activity towards L-phenylalanine and L-tyrosin...

Phenylketonuria is the most prevalent disorder caused by an inborn error in aminoacid metabolism. It results from mutations in the phenylalanine hydroxylase gene. Phenotypes can vary from a very mild increase in blood phenylalanine concentrations to a severe classic phenotype with pronounced hyperphenylalaninaemia, which, if untreated, results in profound and irreversible mental disability. Neo...

The nonselective herbicide glyphosate (n-[phosphonomethyl]glycine) inhibited the light-induced accumulation of phenylpropanoid substances (chlorogenic acid, procyanidin, rutin, anthocyanin) in etiolated buckwheat hypocotyls 90% at 1 millimolar. Structurally related compounds, such as n,n-bis[phosphonomethyl]glycine, aminomethylphosphonate, methylglycine, and iminodiacetate, had little or no inh...

l-Phenylalanine ammonia-lyase has been purified from elicitor-treated alfalfa (Medicago sativa L.) cell suspension cultures using two protocols based on different sequences of chromatofocusing and hydrophobic interaction chromatography. Three distinct forms of the intact enzyme were separated on the basis of affinity for Octyl-Sepharose, with isoelectric points in the range pH 5.1 to 5.4. The n...

Phenylalanine aminomutases (PAMs) are 4-methylideneimidazol-5-one (MIO)-dependent enzymes that catalyze the isomerization of (S)-a-phenylalanine to give (S)or (R)-bphenylalanine, which are precursors in the biosynthesis of various natural products. Several related tyrosine aminomutases (TAMs) have also been characterized. Furthermore, the mechanistically related MIO-dependent phenylalanine, tyr...

We have isolated an enzyme, phenylalanine ammonia-lyase (EC 4.3.1.5.), from the rootlets of Zea mays var. СЕ-IV. After partial purification by fractional salting out with ammonium sulfate and gel chromatography on Sephadex G-100, we obtained a preparation of a 40-fold greater specific activity than that of the crude homogenate. We hbve determined the ap­ parent Michaelis constant and the activa...