The phosphonic analogue of L-phenylalanine, (/?)-(l-am ino-2-phenylethyl)phosphonic acid (A P E P ), inhibits buckwheat phenylalanine ammonia-lyase (PAL) com petitively with a K t value o f 1.5 |iM. The K, value for the (S)-enantiomer is 11.6 jj,M. The corresponding values for the enantiom ers of the phosphonous analogue are 35 and 205 |u.m , respectively. APEP inhibits the light-induced synthe...

So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fr...

Subfractionation of Microsomes, Cell Suspension Culture, PAL, Cinnamic Acid p-Hydroxylase 1. The time course of activity of soluble and microsomal phenylalanine ammonia-lyase (PAL) was studied in dark grown cell cultures of soybean (Glycine max). A distinct activity increase of PAL in the soluble and microsomal fraction occurred prior to the stationary phase of the cell culture. Cinnamic acid p...

Phenylketonuria (PKU) is a genetic metabolic disease in which the decrease or loss of phenylalanine hydroxylase (PAH) activity results in elevated, neurotoxic levels of phenylalanine (Phe). Due to many obstacles, PAH enzyme replacement therapy is not currently an option. Treatment of PKU with an alternative enzyme, phenylalanine ammonia lyase (PAL), was first proposed in the 1970s. However, iss...

In a series of experiment, the response of two contrasting potato species, namely,  Solanum acaule (tolerant) and Solanum tuberosum cv. Agria (intolerant) to salt (80 mM NaCl) and drought stress (15% polyethylene glycol;PEG) was studied in vitro. Furthermore, the role of acetyl salicylic acid (ASA) (1 and 10 µM) in alleviating oxidative stress was investigated. In Agria cultivar, NaCl and PEG r...

The synthesis of substituted d-phenylalanines in high yield and excellent optical purity, starting from inexpensive cinnamic acids, has been achieved with a novel one-pot approach by coupling phenylalanine ammonia lyase (PAL) amination with a chemoenzymatic deracemization (based on stereoselective oxidation and nonselective reduction). A simple high-throughput solid-phase screening method has a...

The flavanone naringenin, an intermediate in flavonoid biosynthesis, was tested for its effect on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, as well as phenolic compounds and lignin contents in soybean (Glycine max L. Merrill) seedlings. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), with or without 0.1 to 0.4 m...

The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of a...

This study was conducted to investigate certain characteristics of the purified Phenylalanine ammonia lyase (PAL). The results revealed that molecular weight 180 kDa by gel filtration and Native-PAGE. optimal pH temperature for enzyme activity were 7 40 °C respectively. stable against values range 6-8. retained 79% its total after incubation one hour at 50 7% (lost 93%) 70 °C. entire completely...