Anaerobic arginine catabolism in Saccharomyces cerevisiae was genetically modified to allow assimilation of all four rather than just three of the nitrogen atoms in arginine. This was accomplished by bypassing normal formation of proline, an unusable nitrogen source in the absence of oxygen, and causing formation of glutamate instead. A pro3 ure2 strain expressing a PGK1 promoter-driven PUT2 al...

Abstract Background CD4+ T cells are associated with pulmonary arterial hypertension (PAH) pathogenesis but mechanistic insights limited. Purpose To identify differential methylation signatures in healthy controls vs PAH and evaluate a putative association the cardiopulmonary hemodynamic profile of affected patients. Methods We used RRBS platform to DNA methylome CLEOPAHTRA clinical trial. Resu...