Irreversible airflow limitation in asthma is associated with airway remodeling in which the differentiation of fibroblasts to myofibroblasts plays a pivotal role. In asthmatic airways, excessive production of reactive nitrogen species (RNS) has been observed. The aim of this study is to evaluate whether peroxynitrite, one of the RNS, can affect the differentiation of fibroblasts to myofibroblas...

Peroxynitrite is a bifaceted reactive species. It can oxidize and nitrate proteins/nucleic acids/lipids and is involved in inflammation, apoptosis, cytotoxicity and autoimmune disorders of unknown etiology, including SLE. In this study, H3 histone exposed to peroxynitrite caused loss of tertiary structure, nitration of tyrosine residues and dityrosine formation. Experimentally produced antibodi...

Oxidative stress may increase production of superoxide and nitric oxide, leading to formation of prooxidant peroxynitrite to cause vascular dysfunction. Having found nitrotyrosine residues, a marker of peroxynitrite action, in placental vessels of preeclamptic and diabetic pregnancies, we determined whether vasoreactivity is altered in these placentas and treatment with peroxynitrite produces v...

PURPOSE . Peroxynitrite is a highly potent reactive oxygen/nitrogen species present in the environment and also endogenously in the eye, that causes a variety of disorders. This study was undertaken to look at the oxidative damage that peroxynitrite causes to the proteins of the lens and the functional consequences thereof. METHODS . Peroxynitrite was allowed to react with alpha-, beta-, and ...

Peroxynitrite toxicity is a major cause of neuronal injury in stroke and neurodegenerative disorders. The mechanisms underlying the neurotoxicity induced by peroxynitrite are still unclear. In this study, we observed that TPEN [N,N,N',N'-tetrakis (2-pyridylmethyl)ethylenediamine], a zinc chelator, protected against neurotoxicity induced by exogenous as well as endogenous (coadministration of NM...

There has been some dispute regarding reaction products formed at physiological peroxynitrite fluxes in the nanomolar range with phenolic molecules, when used to predict the behavior of protein-bound aromatic amino acids like tyrosine. Previous data showed that at nanomolar fluxes of peroxynitrite, nitration of these phenolic compounds was outcompeted by dimerization (e.g. biphenols or dityrosi...

Nitric oxide and superoxide form the unstable compound, peroxynitrite, which can nitrate proteins and compromise function of proinflammatory cytokines at sites of inflammation. Reduced function of proinflammatory proteins such as IL-8, macrophage inflammatory protein-1alpha, and eotaxin suggest an anti-inflammatory effect of nitration. The effects of nitration on anti-inflammatory cytokines suc...

Experiments were designed to test whether nitric oxide (NO) and peroxynitrite trigger delayed coronary endothelial protection induced by preconditioning (PC) in rats. Prolonged ischemia reperfusion markedly reduced the response of isolated coronary arteries to acetylcholine, and this was prevented by PC performed 24 h earlier. The NO synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester (...

(2014) A water-soluble boronate-based fluorescent probe for the selective detection of peroxynitrite and imaging in living cells. This version is made available in accordance with publisher policies. Please cite only the published version using the reference above. A water-soluble boronate-based fl uorescent probe for the selective detection of peroxynitrite and imaging in living cells A water-...