Previously novel histone-derived antimicrobial peptides (HDAPs) were designed based on properties of Buforin 2, a peptide whose activity depends on membrane translocation, and DesHDAP1 and DesHDAP3 showed significant antibacterial activity. Their DNA binding, permeabilization, and translocation abilities were assessed independently and compared to antibacterial activity to determine whether the...

We investigated the inhibitory effects of the antifungal protein (AFP) from Aspergillus giganteus on the growth of several filamentous fungi. For this purpose, the MICs of AFP were determined and ranged from 0.1 micro g/ml for Fusarium oxysporum to 200 micro g/ml for Aspergillus nidulans. The antifungal activity of AFP was diminished in the presence of cations. We were able to show that incubat...

The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under d...

Acute respiration distress syndrome (ARDS) is a typical complication in toxic shock-like syndrome (TSLS) caused by Streptococcus pyogenes. An isolated perfused rat lung model was used to identify the causative agent of ARDS in TSLS in this study. Some crude preparations separated from the culture supernatants of S. pyogenes isolates caused rapid increases in the weight of perfused lungs, indica...

Bax-mediated permeabilization of the outer mitochondrial membrane and release of apoptogenic factors into the cytosol are key events that occur during apoptosis. Likewise, apoptosis is associated with permeabilization of the lysosomal membrane and release of lysosomal cathepsins into the cytosol. This report identifies proteolytically active cathepsin D as an important component of apoptotic si...

Artificial Biomolecular Condensates In article number 2104704, Huaimin Wang and co-workers report a general approach for constructing artificial biomolecular condensates of oligopeptide in living cells. The rationally designed aromatic-capped peptides can reversibly form nanofibrous hydrogel with change the conformation morphologies lysosome. formed undergo swelling lysosome, leading to lysosom...

There is a constant increase in the demand for biomolecules produced by microorganisms for a wide range of commercial applications. Although many such microbial enzymes and proteins are produced extracellularly, an equally high proportion of such potentially useful products are retained within the cells.1–2 One such enzyme, β-galactosidase (EC 3.2.1.23), has found wide application for lactose h...

Trehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a stable and nonreducing disaccharide; can be used as sweetener, stabilizer, humectant; has many applications in the food, pharmaceutical, cosmetic industries. production from maltose catalyzed by trehalose synthase (TreS) simple economically feasible for industrial-scale application. Reducing cost enhancing efficiency of TreS synthesis ...

The ability of cecropin A to permeabilize and depolarize the membranes of Escherichia coli ML-35p bacteria has been compared to its bactericidal activity in an extension of earlier studies performed on synthetic lipid vesicle membranes (L. Silvestro, K. Gupta, J. H. Weiser, and P. H. Axelsen, Biochemistry 36:11452-11460, 1997). Our results indicate that differences in the concentration dependen...