objective: in peripheral blood polymorphonuclear and mononuclear cells nitric oxide (no) could be synthesized by an enzyme called inducible no synthase (inos). inos gene (nos2a) is located on chromosome 17 at position 17q11.2-q12. no is released during inflammatory responses. in the present studies the frequency of nos2a gene polymorphisms and their effects on no production were investigated in...

Background Atazanavir (ATV) is administered at a dose of 300 mg boosted with 100 mg of ritonavir once daily (boosted). However, in the clinical setting use of unboosted ATV can be warranted. Although plasma concentrations are used clinically as a marker of drug exposure, ATV predominantly acts within HIV infected cells and therefore intracellular concentrations may better correlate with therape...

MicroRNAs are non-coding RNAs that negatively regulate mRNA expression and play significant roles in both health and disease. Differential microRNA expression has been used to aid diagnosis and discriminate disease stages. The accuracy and reliability of microRNA expression measurement is of utmost importance. Quantification of microRNA expression in human peripheral blood is commonly detected ...

Methods The HIV inhibitory activity of aqueous and methanolic extracts of S. swartzii was determined on laboratory adapted strains of HIV-1 clades C and A by indirectly measuring reduction in HIV-1 gag p24 antigen levels and inhibition of HIV-1 RT activity in supernatants of virus infected donor peripheral blood mononuclear cell (PBMC) cultures grown in varying concentrations of the extracts. P...

BACKGROUND Peripheral blood mononuclear cells (PBMC) transcribe mRNA for the nonclassical opioid nociceptin/orphanin FQ (N/OFQ) receptor (NOP). We probed for the N/OFQ precursor, pre-pro-N/OFQ (ppN/OFQ). METHODS Using PBMC from 10 healthy volunteers we probed for ppN/OFQ using polymerase chain reaction (PCR) based experimental paradigms. RESULTS In gel-based PCR, we detected amplicons consi...

Peripheral blood mononuclear cells (PBMC) harbored TT virus (TTV) of genotypes (3 and 4) different from those (1 and 2) of free virions in plasma of the same individuals. PBMC may act as a reservoir, and TTV of particular genotypes might have tropism for hematopoietic cells.

Several reports relate the role of different cytokines in the pathogenesis of dengue. Here, we report the production, after trigger with dengue virus, of IL-2 by peripheral blood mononuclear cells of individuals with history of dengue infection 20 years ago.

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Mononuclear cells from human peripheral blood were purified by semicontinuous flow centrifugation (SCFC) using the Haemonetics model 30 blood cell separator; 64% +/- 7% of the mononuclear cells in 600 ml of peripheral blood were collected in a 30-ml volume. Analysis of sequential 5-ml aliquots of the mononuclear cell concentrate revealed that both immunocompetent cells and granulocytic progenit...

The use of marrow aspirates in bone marrow transplantation. clinical evaluation. and experimental studies has prompted the present study of the functional characteristics of mononuclear cells from aspirated (iliac crest) and curettaged (rib) marrow. In this manner. the relative contribution of peripheral blood contamination of aspirated bone marrow cells in investigating and understanding marro...