Brassinosteroids (BRs) play an essential role in plant growth, development, and responses to diverse abiotic stresses. However, previous studies mainly analyzed how exogenous BRs influenced plant physiological reactions to drought stress, therefore, genetic evidences for the endogenous BRs-mediated regulation of plant responses still remain elusive. In this study, a key BRs biosynthetic gene, S...

Introduction: Macro-creatine kinase (Macro-CK) is a complex with longer half-life that leads to elevation of enzyme activity and analytical errors. We present two cases with macro-CK to picture the problem. The aim of the study was to evaluate and compare different methods using polyethylene glycol (PEG) for screening. Methods: Serum samples (n=39) were analysed using different PEG methods PEG6...

A novel, type 1 ribosome-inactivating protein designated charybdin was isolated from bulbs of Charybdis maritima agg. The protein, consisting of a single polypeptide chain with a molecular mass of 29 kDa, inhibited translation in rabbit reticulocytes with an IC50 of 27.2 nm. Plant genomic DNA extracted from the bulb was amplified by PCR between primers based on the N-terminal and C-terminal seq...

Comparative data is presented on glucose and xylose release for enzymatic hydrolysis of solids produced by pretreatment of poplar wood by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid, flowthrough (FT), lime, and sulfur dioxide (SO(2)) technologies. Sugar solubilization was measured for times of up to 72 h using cellulase supplemented with beta-g...

Abstract Drought is a major concern among abiotic stresses in wheat ( Triticum aestivum L.) production. Breeding resistant cultivars are the most effective means to manage drought stress. F 6 recombinant inbred lines (RIL) derived from cross of Berkut/Worrakatta were used identify quantitative trait loci (QTL) for tolerance at germination stage under treatment PEG6000 using 50 K single nucleoti...

Abstract Lipoxygenase (LOX, EC1.13.11.12) catalyzes the oxygenation of polyunsaturated fatty acids and further generates oxylipins via enzymatic or non-enzymatic pathways, thus participating in regulation growth development, stress response, other processes. The objective this research was to characterize function evaluate mechanism lipoxygenase gene AfLOX4 ’s role enhancing drought tolerance. ...

Drought is one of the most important abiotic constraints on agricultural productivity, while global warming leads to occurrence more frequent drought events. Cyclocarya paliurus a multiple-function tree species with medicinal value and timber production, but no information available its tolerance. In this hydroponic experiment, variations in leaf anatomical morphology, chloroplast ultrastructur...

Abstract Phase change material (PCM) experiencing supercooling and phase hysteresis are widely reported in the literature. However, only few studies model analytically such PCM, they mostly focus on either or hysteresis, but rarely both phenomena. Moreover, partial cycles, with an incomplete melting solidification, considered even if these processes occur frequently latent heat thermal energy s...

Ammopiptanthus mongolicus (Maxim. Ex Kom.) Cheng f., a relic tree of the Tertiary period, plays a critical role in maintaining desert ecosystems in the Mid-Asia region. Genome-scale gene expression profiling studies will provide deep insight into the molecular mechanism underlying the drought tolerance of A. mongolicus. In the present study, we investigated the transcriptional changes induced b...