The polymerase chain reaction (PCR) technology offers many benefits to the study of infectious disease, but its use must be restrained by the limits of interpretation of the data generated. Wilborn et al' recently described the results of a PCR-based prospective study of human herpesvirus-6 (HHV-6) infections in bone marrow transplant (BMT) recipients. Their basic conclusions were that HHV6 inf...

Enzyme immunoassay and polymerase chain reaction have become the «gold standard» for detection of biological pathogens. The method amplified – immuno-PCR allows to combine both methods into a single platform preserve their advantages achieve high sensitivity analysis. purpose this work is consider possibilities prospects using PCR-amplified pathogenic agents. Immuno-PCR makes it possible detect...

The introduction of PCR technology to the molecular diagnosis of genetic diseases has increased the speed and range of DNA tests available. Previous analyses of dystrophin gene mutations were time consuming, taking weeks to complete, and used radioisotopic methods. Further developments in DNA amplification and post-amplification techniques have made conventional tube PCR redundant. The rapid me...

Abstract Introduction/Objective Introduction: Standard diagnostic practices for Urinary Tract Infection (UTI) take 2–3 days pathogen identification and antibiotic susceptibility testing leading to delayed diagnosis an increase in healthcare expenditures. The rapid diagnostics tool is highly desirable identify the its antibiotic-resistant genes directly from samples. Our objective validate evalu...

Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...

The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.