Staphylococcus aureus is one of the most important causes of mastitis in dairy cattle. Based on previous research, Staph. aureus genotypes with different pathogenic and contagious properties can cause intramammary infection (IMI) and coexist in the same herd. Our study aimed to compare Staph. aureus strains from herds that differed in IMI prevalence using different molecular approaches such as ...

Clostridium difficile is an emerging threat in hospital environments. To analyse possible transmission and to distinguish between relapse and reinfection a collection of C. difficile isolates, sampled from 162 consecutive episodes of C. difficile infection, were PCR ribotyped. Two ribotypes (001 and 012) were prone to cause nosocomial acquisition. Moreover, ribotype 001 had a tendency to cause ...

AIMS We assessed the veracity of intergenic spacer region 1 (ITS1) ribotyping for the rapid, inexpensive and accurate identification of Brenneria goodwinii and Gibbsiella quercinecans that are associated with acute oak decline (AOD) in the UK. METHODS AND RESULTS Agarose gel electrophoresis and polyacrylamide gel electrophoresis (PAGE) were applied for the typing of ITS1 PCR amplicons from st...

background: clostridium difficile (c. difficile) infection is one of the most important diseases in healthcare facilities and community. ribotypes 027 and 078 are known as hyper-virulent strain of c. difficile in molecular study. pcr-ribotyping is a suitable method to interpret the relation of c. difficile isolated from food and hospital. objectives: in the present study, the clostridim diffici...

A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping. Ribotyping was performed using the fully automated RiboPrinter Microbial Characterization System (Qualicon, Wilmington, Del.). Isolates that were indistinguishable by the Dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (...

BACKGROUND & OBJECTIVE Group A streptococcus (GAS) causes a wide variety of life threatening diseases in developing countries like India. Characterization of GAS is therefore necessary for prevention and control of the disease. Genotypic analysis of GAS is largely lacking from India, therefore an attempt was made to study the genotype distribution of north Indian GAS isolates. METHODS Sixty c...

Within a 1-year period, six surgical-site infections (SSI) caused by Staphylococcus schleiferi were observed in the department of cardiac surgery of Ignatius Hospital, Breda, The Netherlands. Since outbreaks caused by this species of coagulase-negative staphylococci have not been described before, an extensive environmental survey and a case control study were performed in combination with mole...

1) To determine the prevalence of C. difficile contamination in patient rooms and the general environment of a community hospital; 2) Compare C. difficile strains between patients with C. difficile infection (CDI) and the environment using PCR ribotyping; 3) To determine what environmental surfaces are most likely contaminated with C. difficile; 4) Identify factors associated with C. difficile ...