نتایج جستجو برای: pcr multiplex

تعداد نتایج: 186945  

2010
Ayman A. Allam Ahmed amer Kamal Fahmy Ahmed G Siam

Escherichia coli (E.coli) is the most important etiologic agent of childhood diarrhea and represents a major public health problem in developing countries. Aim of this work was to investigate the role of diarrheagenic E.coli in Egyptian children below 5 years age using multiplex PCR and to evaluate multiplex PCR in rapid diagnosis of enteric infections caused by diarrheagenic E.coli strains. Re...

Journal: :Journal of clinical microbiology 2011
Christine C Roberts Ryan Swoyer Janine T Bryan Frank J Taddeo

Real-time human papillomavirus (HPV) type-specific multiplex PCR assays were developed to detect HPV DNA in specimens collected for the efficacy determination of the quadrivalent HPV (type 6, 11, 16, and 18) L1 virus-like particle (VLP) vaccine (Gardasil). We evaluated the concordance between type-specific multiplex HPV PCR and the widely used, commercially available Roche Linear Array genotypi...

2016
S. Ahmed M. S. Abdel - Salam S. S. Hafez W. Nemr

Objective: The study aimed to molecular characterization of Pasteurella multocida local strain using Enterobacterial Repetitive Intergenic Consensus (ERIC), 13 virulence genes and the outer membrane protein (OmpH) gene. Also, development of diagnostic markers by multiplex PCR from investigated genes. Methodology: The ERIC-PCR was used for the detection of the finger print of Pasteurella multoci...

Journal: :Clinical chemistry 2003
Run Zhang Shi Joseph M Morrissey Janet D Rowley

BACKGROUND Characterization of fusion gene transcripts in leukemia that result from chromosome translocations provides valuable information regarding appropriate treatment and prognosis. However, screening for multiple fusion gene transcripts is difficult with conventional PCR and state-of-the-art real-time PCR and high-density microarrays. METHODS We developed a multiplex reverse transcripti...

2012
Daniela Sint Lorna Raso Michael Traugott

1. Multiplex PCR is a valuable tool in many biological studies but it is a multifaceted procedure that has to be planned and optimised thoroughly to achieve robust and meaningful results. In particular, primer concentrations have to be adjusted to assure an even amplification of all targeted DNA fragments. Until now, total DNA extracts were used for balancing primer efficiencies; however, the a...

2012
Mohamed Abbas Shemis Dina Mohamed El-Abd Dalia Ibrahim Ramadan Mohamed Ibrahim El-Sayed Bassem Shenoda Guirgis Mohamed Ali Saber Hassan Mohamed El-Said Azzazy

BACKGROUND At least six HCV (hepatitis C virus) genotypes are unequally distributed worldwide. HCV genotyping guides the selection of treatment regimens and provides important epidemiological markers that enable the outbreak source to be traced and the spread of disease to be controlled. In Egypt, there is an increasing need for cost-effective, fast, and easily performable HCV genotyping assays...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2009

Journal: :Journal of Clinical Microbiology 2003

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