Objetivo. Determinar la circulación del virus Oeste Nilo (VON) en caballos oriente de Yucatán, México. Materiales y métodos. Por conveniencia se estudiaron 184 localizados 23 unidades producción los municipios Tizimín Panabá, Yucatán. De cada caballo obtuvo una muestra suero sanguíneo, cual fue evaluada con un ensayo inmunoabsorción enzimática captura (MAC-ELISA) para detectar inmunoglobulinas ...

background and objectives: invasive and non-invasive techniques are used to diagnose h. pylori infection. some factors influence the choice of a diagnostic test, such as the sensitivity and specificity of the tests, the clinical circumstances and the cost-effectiveness of the testing strategy. the aim of this study was to reveal the relationship between different h. pylori infection diagnosis m...

background the hepatitis delta virus (hdv) was first discovered by rizzetto in 1977 in a patient with chronic hepatitis b virus (hbv) infection. in 1980, it was shown that hdv was an infectious agent responsible for exacerbation of liver disease in patients with hepatitis. objectives the aim of this study was to determine the seroreactivity of, and to molecularly detect, hdv among hemodialysis ...

background: we aimed to develop a pcr-rflp assay based on available sequences of putative vertebrate hosts to iden­tify blood meals ingested by field female sand fly in the northwest of iran. in addition, the utility of pcr-rflp was compared with elisa as a standard method. methods: this experimental study was performed in the insect molecular biology laboratory of school of public health, tehr...

cucumber mosaic virus (cmv) is one of the causal agents of viral diseases that infected mint (mentha spp.). cmv is a member of the cucumovirus genus in  bromoviridae family. the virus is naturally transmitted by aphids in a non-persistent manner.during  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in tehran provi...

In order to overcome some of the limitations of conventional microbiological techniques in the diagnosis of human brucellosis, a simple PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed. After amplification of a 223-bp sequence of a gene that codes for the synthesis of an immunogenetic membrane protein specific for the Brucella genus (BCSP31), the digoxigenin-labeled amplified pro...

Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf sa...