In order to provide much more understanding into the proliferation and differentiation of the epithelia in cholesteatoma, cell cycle associated molecules were studied by anti-PCNA and anti-Ki67, the two well characterized antibodies. In addition, The experiments included expression, localization, and quantification of EGF, TGF, EGF-R in the cholesteatoma. This study demonstrates the presence of...

OBJECTIVE To report on the value and limitations of new MRI techniques in pre- and post-operative MRI of cholesteatoma. The current value of magnetic resonance imaging (MRI) in diagnosing congenital, acquired, and post-operative recurrent or residual cholesteatoma is described. METHODOLOGY AND RESULTS High resolution computed tomography (HRCT) is still considered the imaging modality of choic...

UNLABELLED Bone destruction in chronic otitis media with cholesteatoma is a common phenomenon. Expanding growth of cholesteatoma in the middle ear causes ischemia of the mucosa and bones with granulation tissue production. THE AIM OF THIS STUDY was to assess the expression and distribution of the key regulators of bone destruction: osteoprotegerin (OPG), Receptor Activator for Nuclear Factor ...

To investigate whether keratinocytes proliferate in response to epiregulin produced by subepithelial fibroblasts derived from middle ear cholesteatoma. Tissue samples were obtained from patients undergoing tympanoplasty. The quantitative polymerase chain reaction and immunohistochemistry were performed to examine epiregulin expression and localization in cholesteatoma tissues and retroauricular...

OBJECTIVES/HYPOTHESIS High-resolution optical imaging is an imaging modality that allows visualization of structural changes in epithelial tissue in real time. Our prior studies using contrast-enhanced microendoscopy to image squamous cell carcinoma in the head and neck demonstrated that the contrast agent, proflavine, has high affinity for keratinized tissue. Thus, high-resolution microendosco...

Cholesteatoma is a benign keratinizing and hyper proliferative squamous epithelial lesion of the temporal bone. Epidermal growth factor (EGF) is one of the most important cytokines which has been shown to play a critical role in cholesteatoma. In this investigation, we studied the effects of EGF on the proliferation of keratinocytes and EGF-mediated signaling pathways underlying the pathogenesi...

INTRODUCTION AND AIMS the aim of this study was to determine the certainty of non-echo-planar imaging diffusion-weighted magnetic resonance imaging (non-EPI DW MRI) in the diagnosis of primary and recurrent cholesteatoma in patients with clinical suspicion of cholesteatoma, assessing the sensitivity and specificity of the test in both groups. METHODS Seventy-five patients with clinical suspic...

OBJECTIVES To quantify the barrier sterols and image the lipid structures in the matrix of acquired cholesteatoma and compare the distribution with that found in stratum corneum from normal skin, with the goal to resolve their potential influence on cholesteatoma growth. METHODS High-performance thin-layer chromatography (HPTLC) was used to achieve a quantitative biochemical determination of ...

O Editorial Apreciação do “Plano Nacional de Saúde”, publicado por esta revista em dezembro de 1968 é, sem sombra de dúvida, um texto histórico, pelo menos por dois motivos. Primeiro, por questionar, técnica e politicamente, um documento ministerial numa conjuntura nacional tensa e bastante particular; e, segundo, por espelhar a perspectiva teórica e técnica, vigente na época, do planejamento e...

BACKGROUND: The histopathological characteristics of cholesteatoma are hyperproliferation and differentiation of the epithelium. The proliferative capacity of cholesteatoma was studied through the kinetics of the epithelium of cholesteatoma. The present study was undertaken to compare the rate of proliferation and apoptosis using immunohistochemistry with PCNA antigen and p53 protein in cholest...