The samples of Tagelus plebeius and Iphigenia brasiliana were manually collected on the Cachoeira River estuary region (Ilhéus, BA, Brazil) between August 2005 and August 2006, with a periodicity of 15 days, with 20 animals collected/sampled, performing 500 samples from each species. The animals were measured, eviscerated and kept in solution of Davidson and after 24-30 hours, they were transfe...

In the mid to late 1800s, the light microscopic features of atherosclerosis were discovered as Virchow and others used techniques that were novel at the time for paraffin embedding, sectioning, and staining of tissues.1 From then until the past few years, detailed characterization of atherosclerotic lesions has been limited to autopsied or excised tissues, which display only a single time point...

A sequence of technically reproducible procedures is mandatory to guarantee a proper preservation of tissues and to build up the basis for sound diagnoses. However, while the goal of these procedures was, until recently, to assure only structural (histological and cytological) preservation, an appropriate preservation of antigenic properties and of nucleic acid integrity is now additionally req...

Green fluorescent protein (GFP) and its derivatives are routinely employed as surrogate markers for gene expression and lineage tracing in genetically engineered mice. Tissues from these mice are commonly formalin fixed and paraffin embedded (FFPE) for histological studies. However, this results in inactivation of the natural fluorescence of these proteins, requiring their detection by immunolo...

Formalin fixation, generally followed by paraffin embedding, is the standard and well-established processing method employed by pathologist. This treatment conserves and stabilizes biopsy samples for years. Analysis of FFPE tissues from biopsy libraries has been, so far, a challenge for proteomics biomarker studies. Herein, we present two methods for the direct analysis of formalin-fixed, paraf...

Data on the quality of a modified method for producing multiblocks from conventional blocks of paraffin wax embedded histological material are reported. Originally devised as a way of assessing antibodies for immunohistology, the technique is particularly suited to producing a set of reference and teaching material for use by trainee and senior pathologists.

The aim of this study was to find a reliable method for the detection and identification of fungi in fungus balls of the maxillary sinus and to evaluate the spectrum of fungi in these samples. One hundred twelve samples were obtained from patients with histologically proven fungal infections; 81 samples were paraffin-embedded tissue sections of the maxillary sinus. In 31 cases, sinus contents w...

Fresh or frozen tissue samples will always be the best tissue source for the analysis of nucleic acids and proteins from tissues. However, their long-term storage is expensive and laborious. Much interest has therefore been focused on the question whether the almost infinite resources of formalin fixed and paraffin embedded tissue samples in the archives of pathology and histology departments c...

introduction:in this study, ecg signals have been embedded into medical images to create a novel blind watermarking method. the embedding is done when the original image is compressed using the ezw algorithm. the extraction process is performed at the decompression time of the watermarked image. materials and methods: the multi-resolution watermarking with a secret key algorithm developed in th...