نتایج جستجو برای: paraffin embedded tissue blocks
تعداد نتایج: 1085470 فیلتر نتایج به سال:
This study investigated the use of pressure cooking with different retrieval solutions at variable pHs to determine the optimum protocol for the extraction of high quality DNA from formalin fixed paraffin embedded (FFPE) tissues. Further, the oxidation effect on the archived FFPE tissues in relation to the age of the tissue blocks was also explored. The main aim of the study was to investigate ...
Colorectal cancer is the third most common in men and second women globally. There a marked variation incidence of colorectal carcinoma worldwide, where western countries having high rate compared to others. p53 tumour suppressor gene one intensively studied markers tumours. Two were used, (oncoprotein p53) CEA (carcinoembryonic antigen) study. The 102 cases paraffin-embedded samples processed ...
AIMS To adapt the polymerase chain reaction (PCR) technique of HIV detection to paraffin wax embedded brain tissue and to compare the results with those obtained using frozen tissue. METHODS HIV antigen and HIV proviral DNA were detected in specimens of frontal lobe using immunohistochemistry and PCR, respectively. DNA was extracted from fresh tissue using standard methods whereas the techniq...
Fusarium has recently emerged as an opportunistic pathogen of humans, but the histological differentiation of Fusarium from Aspergillus and Scedosporium is particularly difficult because these fungi may induce similar clinical features and exhibit filamentous development in host tissues. Thus, there is a need to establish rapid and reliable methods that are applicable to pathological diagnoses....
bovine leukemia virus (blv) is a retrovirus causing a chronic leukemia/lymphoma in cattle in many countries around the world. screening for antibodies by elisa has been the primary means of detecting the presence of this virus. it is not yet known if this assay will detect antibodies in all cattle during a concomitant bovine leukemia virus infection. the polymerase chain reaction (pcr) of the t...
Autophagy assures cellular homeostasis, and gains increasing importance in cancer, where it impacts on carcinogenesis, propagation of the malignant phenotype and development of resistance. To date, its tissue-based analysis by immunohistochemistry remains poorly standardized. Here we show the feasibility of specifically and reliably assessing the autophagy markers LC3B and p62 (SQSTM1) in forma...
Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis. Within the SPIDIA project promising results were found in both genomic and proteomic experiments with PAXgene-fixed and paraffin embedded tissue derived biomolecules. But, for this technology to ...
BACKGROUND Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice. METHODS To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chai...
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