The effects of chaotic advection on the in situ assembly of a hierarchal nanocomposite of Poly Amide 6, (nylon 6 or PA6) and platelet shape nanoparticles (NPs) were studied. The assemblies were formed by chaotic advection, where melts of pristine PA6 and a mixture of PA6 with NPs were segregated into discrete layers and extruded into film in a continuous process. The process assembles the nanoc...

Nylon 6 (PA6) reinforced Flax Linum composites were synthesized by melt mix extrusion and molded through Mucell® injection technique for adsorption application removal of Cd(II) Pb(II) metal ions. The structural morphologies all samples evaluated using X-ray diffraction (XRD), Transmittance emission microscopy (TEM) Scanning (SEM), indicating crystallized materials with pore-like cells Thermogr...

In this study, the mechanical performances of neat Polyamide 6 (PA6) polymer, 20wt.% glass fiber reinforced PA6, and 8% SEBS elastomer with 20% PA6 composite were investigated. Composite materials first produced in form granules by using a twin-screw extruder. Later, test samples molded, accordance American Society Testing Materials (ASTM) standards, an injection molding technique. Mechanical p...

sue components such as bone, cartilage, the hematopoietic supporting stroma and adipocytes. Among cell types, the adipocytes are perhaps the most abundant stromal cell phenotype in adult human bone marrow. Generally, adipocytes play an important role in systemic lipid metabolism and also contribute to adiposity, insulin resistance and atherogenesis. Gimble et al. have reviewed the function of b...

The activation and expression of recombination activating genes (RAGs) plays critical roles in V(D)J gene recombination machinery and lymphocyte development. We showed that RAG gene expression was induced in freshly isolated human bone marrow cells and a human lymphoid progenitor cell line, FL8.2.4.4, by coculture on a monolayer of a murine bone marrow-derived stromal cell line, PA6, in the pre...

Accumulating evidence suggests that activin A/erythroid differentiation factor (EDF), a homodimer of beta A chain, is a physiologic hematopoietic factor, particularly of erythroid lineage. Media conditioned by phorbol myristate acetate (PMA)-stimulated murine bone marrow stromal cell lines, MC3T3-G2/PA6 and ST2, contained activin A/EDF, assayed as the activity inducing erythroid differentiation...

CD34 is expressed on human hematopoietic stem and progenitor cells, and its clinical usefulness for the purification of stem cells has been well established. However, a similar pattern of expression for murine CD34 (mCD34) has not yet been determined. Two polyclonal anti-mCD34 antibodies that specifically recognize both endogenous and recombinant murine CD34 were daveloped to characterize the m...

Corneal transparency depends on a unique extracellular matrix secreted by stromal keratocytes, mesenchymal cells of neural crest lineage. Derivation of keratocytes from human embryonic stem (hES) cells could elucidate the keratocyte developmental pathway and open a potential for cell-based therapy for corneal blindness. This study seeks to identify conditions inducing differentiation of pluripo...