Human replication protein A (huRPA) is a multisubunit protein which is involved in DNA replication, repair and recombination processes. It exists as a stable heterotrimer consisting of p70, p32 and p14 subunits. To understand the contribution of huRPA subunits to DNA binding we applied the photoaffinity labeling technique. The photoreactive oligonucleotide was synthesized in situ by DNA polymer...

The ability of Plasmodium falciparum-infected red blood cells (IRBCs) to bind to vascular endothelium, thus enabling sequestration in vital host organs, is an important pathogenic mechanism in malaria. Adhesion of P. falciparum IRBCs to platelets, which results in the formation of IRBC clumps, is another cytoadherence phenomenon that is associated with severe disease. Here, we have used in vitr...

All humans experience anxiety in your life and it is natural that people get anxious when facing stressful situations threatening, but severe and chronic anxiety in the absence of clear cause, is uncommon. Studies show that anxiety in men and a young person are less privileged economic classes and in women, low-income people and the elderly and children and teenagers are more common. When locat...

The genome of Sapovirus (SaV), a causative agent of gastroenteritis in humans and swine, contains either two or three open reading frames (ORFs). Functional motifs characteristic to the 2C-like NTPase (NTPase), VPg, 3C-like protease (Pro), 3D-like RNA-dependent RNA polymerase (Pol), and capsid protein (VP1) are encoded in the ORF1 polyprotein, which is afterwards cleaved into the nonstructural ...

The gene encoding protein p32, the most abundant and immunogenic protein induced by African swine fever virus at early times of infection, has been mapped in the EcoRI C' fragment of the genome of the Vero cell-adapted virus strain BA71V. Sequencing analysis has shown the existence of an open reading frame, named C'204L, encoding 204 amino acids. The protein is phosphorylated in serine residues...

Cancer is a complex, multi-factorial, multi-stage disease and a global threat to human health. Early detection of nature and stage of cancer is highly crucial for disease management. Recent studies have proved beyond any doubt about the involvement of the ubiquitous, myriad ligand binding, multi-functional human protein, hyaluronan-binding protein 1 (HABP1), which is identical to the splicing f...

Previous studies on resting cell metabolism of microorganisms relating to p32 incorporation into the intact cell have been carried out by Hotchkiss (1956) for micrococci, lactobacilli, and streptococci, and by Juni et al. (1948) for yeast. These studies in the main were concerned with turnover of various intracellular phosphoruscontaining compounds during conditions under which the organism was...

The incorporation of radiophosphorus (P”“) into desoxyribonucleic acid (DNA) has been shown to parallel the incidence of mitosis in various tissues (14). In tissues without mitotic activity, such as brain cortex, skeletal muscle, and cartilage, injected P32 was not found in DNA (4). Accordingly, it is generally thought that the presence of P32 in DNA occurs as a result of the synthesis of addit...