endo-derived nitric oxide (no) is synthesized from l-arginine by endothelium nitric oxide synthase (enos). since reduced no synthesis has been implicated in the development of coronary atherosclerosis; we hypothesized that polymorphisms of nos gene might be associated with increased susceptibility to this disorder and coronary artery disease (cad). we studied the 27 base pair tandem repeat poly...

introduction: association between single-nucleotide polymorphisms (snps) in mu opioid receptor gene and drug addiction has been shown in various studies. here, we have evaluated the existence of polymorphisms in exon 3 of this gene in iranian population and investigated the possible association between these mutations and opioid addiction.  methods: 79 opioid-dependent subjects (55 males, 24 fe...

conclusions: it was concluded that increasing the concentration of zinc in the agricultural soil is harmful to beneficial microorganisms and reduces the soil fertility. a 20-ppm zinc concentration in soil is suggested to be optimal. background: zinc is an essential micronutrient used in the form of zinc sulfate in fertilizers in the agriculture production system. nitrogen-fixing microorganisms ...

Objective(s): The aim of this study was to investigate the methylation status and mRNA expression levels of P15, death-associated protein kinase (DAPK), and suppressor of cytokine signaling-1 (SOCS1) genes in multiple myeloma (MM). Materials and Methods: The bone marrow samples of 54 MM patients were collected and the methylation status of the P15, DAPK, and SOCS1 gene promoter regions was dete...

conclusions the analysis of the pro-apoptotic gene to anti-apoptotic gene expression ratio (bad /bcl-xl) confirmed that expression of the pro-apoptotic gene significantly decreased (p value ˂ 0.001) under the ischemia/reperfusion condition. in contrast, the expression of the anti-apoptotic gene increased after administration of fk-506 (p value ˂ 0.001). results the quantitative results of real-...

background: spinal muscular atrophy (sma) is the second most common lethal autosomal recessive disease. it is a neuromuscular disorder caused by degenerative of lower motor neurons and occasionally bulbar neurons leading to progressive limb paralysis and muscular atrophy. the smn1 gene is recognized as a sma causing gene while naip has been characterized as a modifying factor for the clinical s...

the raini cashmere goat is one of the iranian goat breeds with a high economic value on international markets due to its high production and high quality. the bmp15 gene regulates granulose cell proliferation and differentiation by promoting granulosa cell mitosis, suppressing follicle-stimulating hormone receptor expression, and stimulating the kit ligand expression. this gene plays a pivotal ...

background and objectives: phospholipases are a group of enzymes that breakdown phospholipid molecules producing second products. these second products play a diverse role in the cell such as signal transduction and digestion in humans. in this study, the effect of phospholipids on the expression of pld genes of a. fumigatus was investigated. the pld genes of this fungus were also investigated ...

a new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising dna annealing-ligation and augmentation by polymerase chain reaction was introduced. the sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. first, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

background: the hbv-x (hbx) protein is believed to contribute to the development of hcc. however, the molecular mechanisms involved in hbx-mediated hepatocarcinogenesis remain obscure. in this study, the effect of hepatitis b virus x gene and its protein product hbxag on expression of p53 gene in hep g2 cell line was investigated. methods: viral dna extracted from hbv-positive serum and hbx gen...