BACKGROUND Activin A increases production of follicle stimulating hormone (FSH) by inducing transcription of its beta subunit (FSHB). This induction has been studied here in LbetaT2 gonadotropes using transient expression of ovine FSHBLuc (-4741 bp of ovine FSHB promoter plus exon/intron 1 linked to Luc). Several sequences between -169/-58 bp of the ovine FSHB proximal promoter are necessary fo...

Abstract While intracytoplasmic sperm injection (ICSI) is an asset in human Assisted Reproduction Technologies (ART), its outcomes, terms of blastocyst, still unacceptably low ruminants. The picture typically found ICSI derived bovine and ovine embryos asymmetry between a high activation rate, marked by pronuclear development, first cleavage rate. Abnormal centriole function has been indicated ...

background: it has been demonstrated that performing a mock embryo transfer (et) significantly improves the pregnancy rate in ivf cycles. the mock et could be performed prior to the stimulation cycle or immediately before real et. the problem of the first procedure is the possibility of variation in the uterine position and or cervico�uterine angle. objective: the purpose of this study was to d...

background: natural endometrium in frozen-thawed embryo transfer (fet) may have some benefits upon implantation in patients with repeated implantation failure (rif). it might be due to possible differences between natural and stimulated endometrial growth factors and cytokins secretions. objective: the objective of this study was to compare the pregnancy rate of fet on modified natural cycle ve...

BACKGROUND Ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. In the current study, the purpose was to evaluate the effects of ammuniom chloride (AC) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of Bcl-2. METHODS In vitro maturation (IVM) was performed in t...

The objective of the present study was to test the hypothesis that fatty acids are the circulating mediators acting in a pro-inflammatory manner towards activated circulating ovine monocyte/macrophages and neutrophils. Furthermore, whether soya protein hydrolysates (SPH) inhibit the fatty acid-induced increase in the production of pro-inflammatory responses by ovine phagocytes was tested in vit...

The majority of ovarian cancers (>90%) are believed to derive from the ovarian surface epithelium (OSE); a single layer covering the entire surface of the ovary. At ovulation, the OSE cell layer undergoes an inflammatory response, involving cell death and growth, in order to overcome ovarian surface rupture. Abnormalities during these processes are believed to contribute to the development of t...

In the context of a hunt for a postulated hormone that is tissue-mass inhibiting and reproductively associated, there is described probable relatedness to a granin protein. A 7-8 kDa polypeptide candidate (gels/MS) appeared in a bioassay-guided fractionation campaign involving sheep plasma. An N-terminal sequence of 14 amino acids was obtained for the polypeptide by Edman degradation. Bioinform...

Abstract The objective of this study was to use matrix-assisted laser desorption ionisation–time flight mass spectrometry (MALDI-TOF MS) for the identification ovine-associated staphylococci. Presumptive Staphylococcus isolates were recovered from ovine udder surface (US), individual raw milk, bulk tank and cheese samples characterised by conventional phenotypic methods. A total 69 bacterial fu...

The successful application of assisted reproductive techniques (ARTs) in ovine as other mammal species relies on many factors among which the quality semen used. After collection, samples are generally processed for storage (liquid or cryoconservation) before being used insemination vitro embryo production. During liquid process, sperm cells exposed to artificial conditions lead oxidative stres...