To study the role of the signal sequences in the biogenesis of outer membrane proteins, we have constructed two hybrid genes: a phoE-ompF hybrid gene, which encodes the signal sequence of outer membrane PhoE protein and the structural sequence of outer membrane OmpF protein, and a bla-phoE hybrid gene which encodes the signal sequence as well as 158 amino acids of the structural sequence of the...

Outer and inner membranes of Neurospora crassa mitochondria were separated by the combined swelling, shrinking, sonication procedure. Membranes were characterized by electron microscopy and by marker enzyme activities. A red carotenoid pigment was found to be concentrated in the outer membrane. The inner mitochondrial membrane was resolved into about 20 protein bands on polyacrylarnide gel elec...

chlamydia trachomatis is one of the main causes of sexually transmitted diseases (stds) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of chlamydia. in this research the chlamydia major o...

A unique physical feature of Treponema pallidum, the venereally transmitted agent of human syphilis, is that its outer membrane contains 100-fold less membrane-spanning protein than the outer membranes of typical gram-negative bacteria, a property that has been related to the chronicity of syphilitic infection. These membrane-spanning T. pallidum rare outer membrane proteins, termed TROMPs, rep...

introduction: legionnair's disease bacterium is a gram negative, facultative intracellular bacilli which parasitize human alveolar macropages as well as some species of free living amobae. this study was achieved in order to compare the cell wall composition in intracellular bacteria. whole cell and outer membrane proteins and the bacterial lipopolysacharide (lps) were analyzec on sds page syst...