The exotoxin (PE) of Pseudomonas aeruginosa was purified from 50-liter cultures by a simple three-step procedure, yielding 135 mg of essentially homogeneous protein. In Ouchterlony gel diffusion, PE produces a single line which does not interact with a diphtheria toxin-antitoxin precipitin line. The protein has a molecular weight of 66,000, an isoelectric point of 5.1, N-terminal arginine, and ...

Antigen and tumor incidences in BALB/c and C57BL mice after living as weanlings for 5 weeks in cages with mouse mammary tumor virus-infected females were com pared with control BALB/c and C57BL mice living in the same laboratory. All mice were bred continuously, and third-lactation milks were tested for mouse mammary tumor virus antigen by Ouchterlony microimmunodiffusion test. Mammary tumor in...

Two fetus-specific globulins, al and a2·fetoglobulins, were detected in rat fetal serum, and the former was detected in amniotic fluid, using respective monospecific rabbit immune sera. Immunochemical distinction of these two proteins was demonstrated. By polyacrylamide gel disc electrophoresis, al-fetoglobulin was further resolved into fast and slow migrating subcomponents having a similar rea...

Rabbit antiserum against beta-amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with beta-amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of beta-amylase was studied by indirect fluorescence microscopy of thin sectioned germinating rice seed specimens (1-day stage) ...

The rate of diffusion of a protein in solution from a source in agar gel is, within limits, proportional to its original concentration (Ouchterlony, 1949). When plasminogen in biological fluids is allowed to diffuse from a source in agar/fibrinogen gel for a given time, the distance diffused can be ascertained by activation of the plasminogen to plasmin by streptokinase after diffusion has take...

Multiple group-specific (gs) components of the avian leukosis-sarcoma viruses were detected by immunodiffusion (Ouchterlony) tests with sera from hamsters bearing tumors induced by sarcoma viruses and with sera from adult chickens immunized with avian sarcoma or leukosis viruses. Immune hamster sera detected up to four components, whereas chicken sera detected at least one. The hamster and chic...

Ouchterlony immunodiffusion of sonicated Vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. Antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. Tandem two-dimensional immunoelectrophoretic analyses of V. vulnificus antigens from various strains ...

CLINICAL CHEMISTRY, Vol. 35, No. 9, 1989 2017 Fig. 1. (top) High-resolution electrophoresis on agarose gel with beta-migrating restriction of fibrinogen in 1gMwell, and (bottom) immunofixation of plasma, showing crossreactivity of anti-IgM reagent with fibnnogen tion does not clearly identify (2). An isolated light-chain restriction should be corroborated by urine immunoelectrophoresis, and an ...

A liver microsomal protein, previously referred to as preneoplastic antigen, from hyperplastic nodules of rats fed a diet containing 2-acetylaminofluorene has been identified as the enzyme epoxide hydrase [glycol hydro-lyase (epoxideforming), EC 4.2.1.63]. Purified preneoplastic antigen from hyperplastic nodules and purified rat liver microsomal epoxide hydrase are immunochemically identical on...

Evidence from chemical and serological studies indicates that a cellular heteropolysaccharide, also found in lipid extracts and culture filtrate, is present as a group antigen in Mycobacterium tuberculosis H37Ra and in other strains of mycobacteria representing M. kansasii, scotochromogenic and Battey strains. Polysaccharides from the four strains contain the same main sugars, arabinose, and ga...