Zoogloea ramigera, a gram-negative bacterium found in activated sludge, formed volutin granules when excess orthophosphate was added to a phosphate-starved culture. These volutin granules were stainable by hydrogen sulfide after lead acetate treatment and extractable by N-perchloric acid but were not adsorbed by activated charcoal. They appeared to consist of inorganic polyphosphate. Optimum gr...

Remineralization of labile organic nitrogen is a key part cycling in seawater, yet factors controlling this process remain poorly understood. The degradation and fate amino acids coastal waters were investigated with incubation experiments using 15N-labeled alanine (Ala). Ala, including the release NH4+ through remineralization, subsequent removal via assimilation nitrification, formation refra...

Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cer...

Linolenate has been shown [I] to inhibit photosynthesis in intact spinach chloroplasts by inhibiting the influx of orthophosphate and the efflux of 3-phosphoglyceraldehyde. The inhibitory effect of linolenate was shown to depend on the level of the internal pool of metabolites [2] as a result of experiments which on the one hand used chloroplasts from preilluminated leaves or chloroplasts which...

Thiobmillus thioparus is an autotrophic microorganism that obtains the energy required for growth by the oxidation of reduced inorganic sulfur compounds to sulfate. When it is grown on high concentrations of thiosulfate, the organism accumulates elemental sulfur and produces sulfate. The pathway of thiosulfate oxidation has been essentially unknown; however, it has been postulated that tetrathi...

reaction, which occurs under mild conditions and at low concentrations of reactants, is absolutely dependent on the presence of bivalent metal ions, and is stimulated by univalent metal ions. The most effective bivalent metal ion was found to be Ca++. When Mn++ ion was used to satisfy the bivalent metal ion requirement, the most effective univalent metal ion was found to be Kf. The reaction was...

Activation by orthophosphate of a plant invertase from root nodules of Lupinus luteus L. has been demonstrated. The activation affects an increase in maximum velocity (V(max)) of the reaction. Activation was also achieved with a number of similar anions and it has been possible to infer a broad classification of anions capable of serving as activators. The possibility of orthophosphate activati...

Both enzymatic hydrolysis and solution (31)P nuclear magnetic resonance (NMR) spectroscopy have been used to characterize P compounds in animal manures. In this study, we comparatively investigated P forms in 0.25 M NaOH/0.05 M EDTA extracts of dairy and poultry manures by the two methods. For the dairy manure, enzymatic hydrolysis revealed that the majority of extracted P was inorganic P (56%)...

There is considerable concern about pollution of surface waters with P. Although most of the research has focused on inorganic P in surface runoff, it has recently become possible to easily follow the fate of soluble organic P forms in soils and waters. Two experiments were performed to compare the relative mobility and soil fixation affinity of orthophosphate monoesters, orthophosphate diester...