Following earlier work demonstrating the utility of Orc as a means of specifying and reasoning about grid applications we propose the enhancement of such specifications with metadata that provide a means to extend an Orc specification with implementation oriented information. We argue that such specifications provide a useful refinement step in allowing reasoning about implementation related is...

BACKGROUND Laparoscopic radical cystectomy (LRC) and robot-assisted radical cystectomy (RARC) are increasingly popular, but high-level evidence for these techniques remains lacking. OBJECTIVE To compare the outcomes of patients undergoing open radical cystectomy (ORC), RARC, and LRC. DESIGN, SETTING, AND PARTICIPANTS From March 2009 to July 2012, 164 patients requiring radical cystectomy fo...

Despite increasing data on the properties of replication origins, molecular mechanisms underlying origin recognition complex (ORC) positioning in the genome are still poorly understood. The Su(Hw) protein accounts for the activity of best-studied Drosophila insulators. Here, we show that Su(Hw) recruits the histone acetyltransferase complex SAGA and chromatin remodeler Brahma to Su(Hw)-dependen...

Replicators are genetically defined elements within chromosomes that determine the location of origins of DNA replication. In the yeast Saccharomyces cerevisiae, the ARS1 replicator contains multiple functional DNA elements: an essential A element and three important B elements--B1, B2, and B3. Functionally similar A, B1, and B2 elements are also present in the ARS307 replicator. The B3 element...

www.sciencemag.org (this information is current as of February 11, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/323/5915/789 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/323/5915/789/DC1 can be fou...

In Saccharomyces cerevisiae, transcriptionally silent heterochromatin at HML and HMR loci is established by silencers that recruit SIR complex and promote its propagation along chromatin. Silencers consist of various combinations of two or three binding sites for origin recognition complex (ORC), Abf1 and Rap1. A single ORC, Abf1 or Rap1 site cannot promote silencing, but can enhance silencing ...

The origin recognition complex (ORC) is a conserved heterohexamer required for the formation of pre-replication (pre-RC) complexes at origins of DNA replication. Many studies of ORC subunits have been carried out in transformed human cell lines but the properties of ORC in primary cells have not been addressed. Here, we compare the expression levels and chromatin-association of ORC subunits in ...

Organic Rankine Cycle (ORC) is named for its use of an organic, high molecular mass fluid that boils at a lower temperature than the water. Among many well-proven technologies, the ORC is one of the most favorable and promising ways for low-temperature applications. In comparison to water, organic fluids are advantageous when the plant runs at low temperature or low power. The ORC is scalable t...

Organic Rankine Cycle (ORC) power systems are nowadays an option for local and domestic cogeneration of heat and electric power. Very interesting are micropower systems for heat recovery from low potential (40–90 °C) waste and renewable heat sources. Designing an ORC system dedicated to heat recovery from such a source is very difficult. Most important problems are connected with the selection ...